FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1711643
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTACGGAAGCTCACCGAAGTTCGGCCAAGGGACCAAGGTGGAAATCAAAAGAACTGTGG53150.31052035967780667No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43790.2558360592717056No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37350.21821139104357626No Hit
CAGTAATACACTGCAGAATCTTCAGGCTCCAGTCTGGCGATGGTGAGACTGAAGTCTGTCCCAGACCCACTGCCA36530.21342067241825544No Hit
GTGTATTACTGTCAGCAGTACGGAAGCTCACCGAAGTTCGGCCAAGGGAC33380.19501730208927914No Hit
GTCTCCAGGCAGCCTGTCTTTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGGCCAGTCAGAGAATTAGCAG31970.1867796029896421No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA29620.17305010449024708No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29360.17153109614563317No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28800.16825938586492628No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTT26340.15388722998896381No Hit
GTCTGGGACAGACTTCAGTCTCACCATCGCCAGACTGGAGCCTGAAGATTCTGCAGTGTATTACTGTCAGCAGTA25920.15143344727843364No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24460.1429036311894478No Hit
GTAATACACTGCAGAATCTTCAGGCTCCAGTCTGGCGATGGTGAGACTGAAGTCTGTCCCAGACCCACTGCCA23550.13758710198329907No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22750.1329132301537178No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22670.13244584297075968No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21630.126369809592304No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21130.12344863969881568No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT20900.12210490154781109No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19600.11450985982474149No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18830.1100112581887695No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18810.10989441139302998No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18750.10954387100581137No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18690.10919333061859278No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18360.10726535848889049No Hit
CTCCCAGACTCCTCATCTATGGTGTTTCCACCAGGGCCACTGGCATCCCA18340.10714851169315097No Hit
GTACGGAAGCTCACCGAAGTTCGGCCAAGGGACCAAGGTGGAAATCAAAAGAACTGTGGCTGCACCATCTGTCTT18030.10533738635918823No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17720.10352626102522547No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17170.10031297414238834No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)