FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004692368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1711643
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTACGGAAGCTCACCGAAGTTCGGCCAAGGGACCAAGGTGGAAATCAAAAGAACTGTGG51990.3037432455249138No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44410.2594583099396311No Hit
CAGTAATACACTGCAGAATCTTCAGGCTCCAGTCTGGCGATGGTGAGACTGAAGTCTGTCCCAGACCCACTGCCA35020.20459873933992076No Hit
GTGTATTACTGTCAGCAGTACGGAAGCTCACCGAAGTTCGGCCAAGGGAC34570.20196968643578128No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33960.19840585916572556No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29630.17310852788811687No Hit
GTCTCCAGGCAGCCTGTCTTTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGGCCAGTCAGAGAATTAGCAG29390.17170636633924247No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29060.1697783942095402No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28420.16603929674587517No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27460.1604306505503776No Hit
GTCTGGGACAGACTTCAGTCTCACCATCGCCAGACTGGAGCCTGAAGATTCTGCAGTGTATTACTGTCAGCAGTA26940.15739263386114977No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTT25090.14658430525524305No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24490.1430789013830571No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23570.13770394877903863No Hit
GTAATACACTGCAGAATCTTCAGGCTCCAGTCTGGCGATGGTGAGACTGAAGTCTGTCCCAGACCCACTGCCA23390.13665232761738283No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT20340.11883319126710418No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20320.11871634447136466No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20090.11737260632036003No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19350.11304927487799735No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18710.10931017741433233No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18650.10895963702711371No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18330.1070900882952812No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17740.10364310782096502No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17410.10171513569126273No Hit
GTACGGAAGCTCACCGAAGTTCGGCCAAGGGACCAAGGTGGAAATCAAAAGAACTGTGGCTGCACCATCTGTCTT17370.10148144209978366No Hit
CTCCCAGACTCCTCATCTATGGTGTTTCCACCAGGGCCACTGGCATCCCA17280.10095563151895576No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17220.10060509113173717No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17200.10048824433599762No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)