FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072382
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATAGCCGTGTCCTCGTCTCTCAGGCTGTCCATTTGCAGATAAA30630.2856258310937707No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22880.21335680755551661No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21430.19983550637739164No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20840.19433373555318906No Hit
ATATATTACTGTGCGAGAGATTCCCCTGCGAATTGTGCTAGTTCCAGTTG19050.1776419223746762No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17870.16663838072627105No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17810.16607887860855555No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16620.1549820866071978No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA15510.14463129742946076No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15320.14285954072336163No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14850.13847677413459009No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC14300.13334800472219788No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13680.12756648283913755No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAAATCTAGTCAAAGCCTCGTA13580.12663397930961168No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13430.1252352240153229No Hit
GTCTTGGAGGCTGGCCTGGCCTCTGCTGAAGCCAACTCAAGTAGGTGTTTCCATCACTGTGTACGAGGCTTTGA12780.11917395107340482No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT12560.11712244330844793No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC12420.11581693836711171No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11970.11162067248424534No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11880.11078141930767207No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11630.10845016048385743No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC11600.10817040942499967No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11600.10817040942499967No Hit
GTGATGGAAACACCTACTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTCATTTACAAGATTT11350.10583915060118501No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT11220.10462689601280141No Hit
CTCCAAGACTCCTCATTTACAAGATTTCCAACCGGTACTCTGGGGTCCCC11060.10313489036556003No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10870.1013631336594609No Hit
GGCCAGGCCAGCCTCCAAGACTCCTCATTTACAAGATTTCCAACCGGTAC10740.10015087907107728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)