FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072382
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30260.28217556803452504No Hit
GTAATATATAGCCGTGTCCTCGTCTCTCAGGCTGTCCATTTGCAGATAAA26600.24804593885387857No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20990.19573249084747787No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20240.1887387143760339No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18610.1735389068447624No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18580.17325915578590464No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17950.16738438354989174No Hit
ATATATTACTGTGCGAGAGATTCCCCTGCGAATTGTGCTAGTTCCAGTTG17820.16617212896150813No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17500.16318811766702537No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16210.1511588221361418No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15450.14407179531174527No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13520.12607447719189618No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13370.12467572189760738No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12910.12038620566178843No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12470.11628319013187464No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12430.11591018872006431No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAAATCTAGTCAAAGCCTCGTA12380.11544393695530138No Hit
GTCTTGGAGGCTGGCCTGGCCTCTGCTGAAGCCAACTCAAGTAGGTGTTTCCATCACTGTGTACGAGGCTTTGA12110.11292617742558156No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11940.1113409214253876No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11720.10928941366043071No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11610.10826365977795226No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11580.1079839087190945No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC11480.10705140518956864No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11220.10462689601280141No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11020.1027618889537497No Hit
GTGATGGAAACACCTACTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTCATTTACAAGATTT10730.10005762871812471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT901.8713054E-645.9576670
GGTATCA13300.045.4672471
CCTAACG705.18412E-1043.656792
CTAACGC705.18412E-1043.646613
GTGGTAT3200.038.2193071
GTATCAA35150.038.177021
TCCGAGA5100.035.14409670
TGGTATC3650.034.4204252
GTCTATA400.002799899333.9727171
ATCAACG41700.032.1563763
TATCAAC41750.032.044032
TCAACGC41950.031.9617614
CAACGCA43100.031.1906175
AACGCAG43950.030.8963496
CGTGCCA500.00535788329.7544767
CGCAGAG47200.028.7689538
ACGCAGA47350.028.6778167
TAACGGC1452.3646862E-1128.41241836
TCGTAGC605.951023E-428.2960682
TTAACGG1353.2923708E-1027.93969235
Produced by FastQC (version 0.11.5)