FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824492
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT21060.2554300102366063No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20500.24863794918568038No Hit
CCATTATACTGGGACCACCTACTACAATCCGTCCCTCAGGGGTCGAGTCA19000.23044492851355744No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18780.2277766188149794No Hit
GCCTTGGGCTGACTCAGGACGGTCAGCCTGGTCCCTCCGCCGAGAACCAC17550.2128583418638386No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17170.20824944329356743No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16560.20085094822023744No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC16370.19854649893510184No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15870.1924821587110609No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15780.1913905774707335No Hit
GTCATGGATCTCATGTGCAAGAAATTGAAGCACCTGTGGTTCTTCGTCCTGCTGGTGGCGGCTCCCAGATCGGTC15510.18811583374975135No Hit
GAAATAAACAGCCGTGTCTGCGGCGGTCACAGAGATCAGGTTCAGGGAGA15070.1827792143525953No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14950.18132377269882546No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG14950.18132377269882546No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGCCTGGTCCCTC13570.16458619368047234No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCACCAGTGACGTT13450.1631307520267025No Hit
ACCTACTACAATCCGTCCCTCAGGGGTCGAGTCACCATATCAGTGCACACGTCCAAGAAGCAGTTCTCCCTGAAC13370.16216045759085593No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12850.15585354375785332No Hit
GTCCTGTCCCAGCTGCAGCTGCGGGAGTCGGGCCCAGGACTGCTGAAGCCTTCGGACACCCTGTCCCTCACTTGC12400.15039563755621643No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12320.14942534312036987No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12180.1477273278576384No Hit
GTATAATGGACACTGGCAATCCACTCCAGCCCCTTCCCGGGGGGCCGGCGGATCCAGGCCCAGTAGTCATCATTA12100.14675703342179183No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12040.14602931259490692No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11800.14311842928736723No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11780.1428758556784056No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11750.14251199526496316No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11440.13875210432605775No Hit
CTCCTGCACTGGAACCACCAGTGACGTTGGTGGTTATAATTATGTCTCCTGGTACCAGCAGCACCCAGGCAAAGC11300.13705408906332625No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11190.13571993421403725No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11190.13571993421403725No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11100.13462835297370987No Hit
GTTTATTTCTGTGCGAGACATGGCATGCATAATTGGGACATTGACTACTGGGGCCAGGGAACCCTGGTCACCGT11060.1341432057557866No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10950.13280905090649758No Hit
CTACTGGGCCTGGATCCGCCGGCCCCCCGGGAAGGGGCTGGAGTGGATTGCCAGTGTCCATTATACTGGGACCAC10740.13026202801240036No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10460.12686599748693742No Hit
GTAGTAGGTGGTCCCAGTATAATGGACACTGGCAATCCACTCCAGCCCCTTCCCGGGGGGCCGGCGGATCCAGGC10450.1267447106824566No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10310.12504669541972513No Hit
GTCGATCACCATCTCCTGCACTGGAACCACCAGTGACGTTGGTGGTTATAATTATGTCTCCTGGTACCAGCAGCA10270.12456154820180185No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG10250.12431897459284019No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC9990.12116551767633889No Hit
CCATATCAGTGCACACGTCCAAGAAGCAGTTCTCCCTGAACCTGATCTCT9980.12104423087185806No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9970.12092294406737725No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9960.12080165726289643No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9960.12080165726289643No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9930.12043779684945398No Hit
TCTCTGGGCTCCAGGCTGACGACGAGGCAGATTATTTCTGTATCTCCTATACAAGCACCAGCACTGCCGTGGTT9880.11983136282704987No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9750.11825463436879921No Hit
GTATAGGAGATACAGAAATAATCTGCCTCGTCGTCAGCCTGGAGCCCAGA9650.11704176632399102No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG9640.11692047951951019No Hit
CCTTGGGGCTGGTCGGGGATGCTGGTGAGACGGTGACCAGGGTTCCCTGG9540.115707611474702No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT9330.11316058858060478No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9310.11291801497164315No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9310.11291801497164315No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9270.11243286775371986No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9240.1120690073402774No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT9140.11085613929546921No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGGTGAGACGGTGACCAGGGTTCCCTGG9090.1102497052730651No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC9070.11000713166410347No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT8970.10879426361929527No Hit
ATACTGGGACCACCTACTACAATCCGTCCCTCAGGGGTCGAGTCACCATA8950.10855169001033364No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG8850.10733882196552545No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8840.10721753516104461No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8800.10673238794312134No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8710.10564080670279397No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT8670.10515565948487068No Hit
CACCATATCAGTGCACACGTCCAAGAAGCAGTTCTCCCTGAACCTGATCT8630.1046705122669474No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8400.10188091576388855No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG8350.10127448174148444No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA8340.10115319493700363No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC8330.10103190813252282No Hit
CTCCTATACAAGCACCAGCACTGCCGTGGTTCTCGGCGGAGGGACCAGGCTGACCGTCCTGAGTCAGCCCAAGG8290.10054676091459952No Hit
GTCTCTGGTGGCTCCATCAGCAGTAATGATGACTACTGGGCCTGGATCCG8290.10054676091459952No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC8260.10018290050115707No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)