FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824492
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73380.8900025712802551No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18200.22074198415509189No Hit
CCATTATACTGGGACCACCTACTACAATCCGTCCCTCAGGGGTCGAGTCA17610.21358606269072353No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC17210.2087345905114907No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16300.19769749130373612No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16240.1969697704768512No Hit
GTCATGGATCTCATGTGCAAGAAATTGAAGCACCTGTGGTTCTTCGTCCTGCTGGTGGCGGCTCCCAGATCGGTC16010.19418017397379234No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG15940.1933311663424266No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15580.1889648413811171No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15560.18872226777215548No Hit
GCCTTGGGCTGACTCAGGACGGTCAGCCTGGTCCCTCCGCCGAGAACCAC15070.1827792143525953No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14320.17368270401653382No Hit
GAAATAAACAGCCGTGTCTGCGGCGGTCACAGAGATCAGGTTCAGGGAGA14020.17004409988210922No Hit
ACCTACTACAATCCGTCCCTCAGGGGTCGAGTCACCATATCAGTGCACACGTCCAAGAAGCAGTTCTCCCTGAAC13200.16009858191468201No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12820.15548968334441088No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12500.15160850560102462No Hit
GTATAATGGACACTGGCAATCCACTCCAGCCCCTTCCCGGGGGGCCGGCGGATCCAGGCCCAGTAGTCATCATTA12150.14736346744419596No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGCCTGGTCCCTC12060.14627188620386858No Hit
GTCCTGTCCCAGCTGCAGCTGCGGGAGTCGGGCCCAGGACTGCTGAAGCCTTCGGACACCCTGTCCCTCACTTGC12050.14615059939938777No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCACCAGTGACGTT11970.14518030496354117No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11810.14323971609184807No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11710.14202684804703988No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11520.1397223987619043No Hit
TCTCTGGGCTCCAGGCTGACGACGAGGCAGATTATTTCTGTATCTCCTATACAAGCACCAGCACTGCCGTGGTT11400.13826695710813444No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC11350.13766052308573037No Hit
GTTTATTTCTGTGCGAGACATGGCATGCATAATTGGGACATTGACTACTGGGGCCAGGGAACCCTGGTCACCGT11150.13523478699611396No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11040.13390063214682496No Hit
CTCCTGCACTGGAACCACCAGTGACGTTGGTGGTTATAATTATGTCTCCTGGTACCAGCAGCACCCAGGCAAAGC11020.1336580585378633No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10860.1317174696661702No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10850.1315961828616894No Hit
GTCGATCACCATCTCCTGCACTGGAACCACCAGTGACGTTGGTGGTTATAATTATGTCTCCTGGTACCAGCAGCA10780.13074717523032364No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10620.12880658635863051No Hit
GTAGTAGGTGGTCCCAGTATAATGGACACTGGCAATCCACTCCAGCCCCTTCCCGGGGGGCCGGCGGATCCAGGC10610.1286852995541497No Hit
CCTTGGGGCTGGTCGGGGATGCTGGTGAGACGGTGACCAGGGTTCCCTGG10500.1273511447048607No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10300.1249254086152443No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG10290.12480412181076347No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10240.12419768778835938No Hit
CTACTGGGCCTGGATCCGCCGGCCCCCCGGGAAGGGGCTGGAGTGGATTGCCAGTGTCCATTATACTGGGACCAC9930.12043779684945398No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9900.12007393643601151No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9800.1188610683912033No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9590.11631404549710611No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG9320.11303930177612397No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT9310.11291801497164315No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9200.11158386012235413No Hit
GTCCCTCACTTGCCTTGTCTCTGGTGGCTCCATCAGCAGTAATGATGACTACTGGGCCTGGATCCGCCGGCCCCC9180.11134128651339248No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGGTGAGACGGTGACCAGGGTTCCCTGG9150.11097742609995002No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9040.10964327125066102No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9030.10952198444618018No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8940.10843040320585283No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG8770.10636852752967887No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8620.10454922546246659No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8590.10418536504902412No Hit
GTCTCTGGTGGCTCCATCAGCAGTAATGATGACTACTGGGCCTGGATCCG8580.1040640782445433No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8560.10382150463558167No Hit
CTCCTATACAAGCACCAGCACTGCCGTGGTTCTCGGCGGAGGGACCAGGCTGACCGTCCTGAGTCAGCCCAAGG8530.10345764422213921No Hit
CCCTGTCCCTCACTTGCCTTGTCTCTGGTGGCTCCATCAGCAGTAATGATGACTACTGGGCCTGGATCCGCCGGC8420.1021234893728502No Hit
ATACTGGGACCACCTACTACAATCCGTCCCTCAGGGGTCGAGTCACCATA8420.1021234893728502No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG8420.1021234893728502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)