FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1422397
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGCGGCGGTCATGGAGCTCAGCTTCAGGGAAA71670.5038677668752114No Hit
GTATATTACTGTGCGAGACAACTCCTAACATACGATCTTTGGAGTGCTTC51960.3652988581950046No Hit
GTCTCTGGTGGCTCCATCAGTTTTAGTGGTTATTACTGGGGCTGGATCCG39970.28100452967772005No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38660.27179472397649884No Hit
CTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC34380.24170467176182178No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32960.23172152359713918No Hit
GCTTAATAGTTATCCTCTCACTTTCGGCGGAGGGACCAAAGTGGAGATCAAACGAACTGTGGCTGCACCAT31010.21801227083577931No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26120.18363368314190764No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23900.1680262261520518No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTTTTAGTGGTTATTACTGGG23750.1669716682473318No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23400.16451103313631849No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22400.15748064710485188No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22400.15748064710485188No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22000.15466849269226524No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21700.15255937688282525No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21350.15009874177181196No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20820.14637263717513466No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20390.14334957118160402No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19550.13744404691517206No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19500.13709252761359875No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18990.13350703073755077No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18960.13329611915660677No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT18720.13160882650905478No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCTCCGCCGAAAGTG18560.13048396474402013No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAACAG18560.13048396474402013No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17680.12429722503632952No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17400.12232871694751886No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17110.12028990499839355No Hit
GGGCATTAACAGTCATTTAGCCTGGTATCAGCAAAAATCAGGGAAAGCCCCTAAGCTCCTGATCTATGATGCATC16660.11712623128423358No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16580.11656380040171625No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16190.11382194984944427No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGAAGGAAGCA16060.11290799966535363No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15860.11150192245906032No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15850.11143161859874563No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15710.11044736455434032No Hit
CAGTAATATACAGCCGTGTCTGCGGCGGTCATGGAGCTCAGCTTCAGGGA15530.10918189506867633No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15470.10876007190678834No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15400.10826794488458567No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15370.10805703330364166No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15100.10615882907514568No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15080.10601822135451636No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14890.10468244800853771No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14820.10419032098633503No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14690.10327637080224437No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14670.10313576308161505No Hit
GATCTATGATGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA14430.10144847043406308No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14400.10123755885311907No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14310.10060482411028708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT15750.065.7236970
TACGTAT701.9346267E-550.6432570
CTACGGA16300.042.20363269
ATACGGA557.407359E-540.34716469
GTGGTAT3250.039.848041
GTATCAA31900.039.5291821
GACGTGT17300.035.69328362
CACGTTA400.002338990335.24258456
GTGTCTA16900.035.22625765
ATCAACG36100.034.8088653
TATCAAC36200.034.716372
TCAACGC36300.034.5256964
TCTACGG17850.034.5034668
TGTCTAC17600.034.18047766
ACGTGTC17400.034.1604863
CAACGCA37150.033.8274155
GGACGTG17900.033.8213761
TGTGCGA18550.033.78001810
ATATTAC19500.033.530293
CTGTGCG19150.033.2551429
Produced by FastQC (version 0.11.5)