FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150807
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA51960.45150924525137576No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36740.3192542276854416No Hit
GTATGATAGTTATTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT33710.29292487793348493No Hit
GTGTATTACTGTGCGAGACACACTATGACTACGCCATTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTC32360.2811939795291478No Hit
CTATCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC31960.2777181577797146No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31220.2712878875432631No Hit
CACTATGACTACGCCATTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCC26710.2320979973184035No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25740.22366912957602794No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25630.22271327859493384No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC24650.21419751530882244No Hit
GTCATAGTGTGTCTCGCACAGTAATACACAGCCGTGTCTGCGGCGGTCAC23780.20663760300380515No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGA23780.20663760300380515No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22110.19212604719992143No Hit
ATCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT21930.1905619274126765No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTGTTAGTAACTGGCTGGCCTGGTATCAGCAGAGA21370.18569577696346998No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21320.18526129924479084No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20480.17796207357098107No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20120.17483383399649116No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20010.17387798301539703No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19970.1735304008404537No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19900.17292213203430287No Hit
CCTTCCACCCTGTCTGCCTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTGTTAGTAACTGG19480.169272519197398No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18780.16318983113588986No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18250.15858436731789083No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA18110.1573678297055892No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17750.1542395901310993No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17060.148243797613327No Hit
GCCTCTGGTGGCTCCATCAGCAGTAGCAGTTACTACTGGGGCTGGATCCG16630.14450728923268627No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCTTATCCCTAGACACGTCCAGGAA16610.14433349814521462No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16510.1434645427078563No Hit
ATAATAGATAGTCCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGTAACTGCTA16480.14320385607664882No Hit
CACCTGCACTGCCTCTGGTGGCTCCATCAGCAGTAGCAGTTACTACTGGG16460.14303006498917714No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16450.14294316944544133No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15790.13720806355887652No Hit
CAGTATGATAGTTATTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT15350.13338465963449997No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC15010.1304302111474817No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14830.12886609136023677No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTGTTAGT14800.1286054047290293No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT14780.12843161364155764No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14630.12712818048552016No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14610.1269543893980485No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14340.12460820971718106No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14300.12426062754223774No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14100.12252271666752114No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13960.12130617905521952No Hit
ATCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC13870.12052411916159704No Hit
GTATTACTGTGCGAGACACACTATGACTACGCCATTCGACCCCTGGGGCC13800.11991585035544623No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13650.11861241719940877No Hit
TCTCTAAGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCACTGGATCTGGGACAGAATTCACT13470.11704829741216383No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13450.11687450632469215No Hit
CTATAATAGATAGTCCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGTAACTGCTA13440.11678761078095631No Hit
GTTAGTAACTGGCTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAA13430.1167007152372205No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13400.11644002860601299No Hit
GACCAGGGAAAGCCCCTAAACTCCTGATCTCTAAGGCGTCTACTTTAGAA13220.11487590881876805No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13220.11487590881876805No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13190.11461522218756054No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13160.11435453555635305No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT13040.1133117890315231No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12940.11244283359416479No Hit
GAGTGAATTCTGTCCCAGATCCAGTGCCGCTGAACCTTGATGGGACCCCA12920.11226904250669313No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12810.111313191525599No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCTTATCCCTAGACACGTCCAGGAACCACTTCTCCCTGAAGCTGA12780.11105250489439149No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12630.10974907173835403No Hit
GAGTGTTAGTAACTGGCTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAACTCCTGATCTCTAAGGCGT12570.10922769847593906No Hit
GTCAGAGTGTTAGTAACTGGCTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAACTCCTGATCTCTAAGG12520.1087932207572599No Hit
GGTATCAGCAGAGACCAGGGAAAGCCCCTAAACTCCTGATCTCTAAGGCGTCTACTTTAGAAAGTGGGGTCCCAT12470.10835874303858076No Hit
GTCTGCCTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA12360.10740289205748663No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12320.1070553098825433No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12160.10566498118277001No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12150.10557808563903417No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12140.10549119009529834No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11970.10401396585178922No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11930.1036663836768459No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11780.10236295052080845No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC150.00246057368.245063
TAGGGAT13500.058.22252770
CCGTCGT200.007131325552.16296447
TGTCCGT200.007598079351.3332531
TCGTATG2100.050.88208440
CGTATGC2200.050.20652841
CGAGACG2450.050.15667323
ATGCCGT1950.047.9635244
ATCTCGT1900.047.01678537
GTATGCC2450.045.1429442
ATCTCCG2800.045.08657510
CCGTCTT1900.043.92670447
ACGAGAC2900.043.5488922
CGAGCCC2850.043.1021515
CCCACGA2950.042.80147619
CACGAGA3000.042.09543221
TACATCT2200.042.08061634
AGACGGA2950.041.66097325
TACACAT3200.041.586845
CATCTCG2150.041.50794636
Produced by FastQC (version 0.11.5)