FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150807
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA49850.43317428552311554No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38630.3356774854515136No Hit
GTGTATTACTGTGCGAGACACACTATGACTACGCCATTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTC33860.2942283110895224No Hit
GTATGATAGTTATTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT33760.2933593556521641No Hit
CTATCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC29710.2581666604391527No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27760.24122202941066576No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25810.22427739838217875No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25700.22332154740108465No Hit
CACTATGACTACGCCATTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCC25540.22193121870131136No Hit
GTCATAGTGTGTCTCGCACAGTAATACACAGCCGTGTCTGCGGCGGTCAC24220.2104610069281817No Hit
ATCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT23870.20741966289742764No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23730.20620312528512602No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22650.1968184065616563No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20860.18126410423294262No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20830.18100341760173513No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGA20190.17544210280264197No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTGTTAGTAACTGGCTGGCCTGGTATCAGCAGAGA19290.16762150386641722No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19050.16553601081675728No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18320.15919263612404166No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18250.15858436731789083No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17950.15597750100581592No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17540.15241478371264686No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17490.1519803059939677No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA17310.15041618620672276No Hit
CCTTCCACCCTGTCTGCCTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTGTTAGTAACTGG17300.15032929066298692No Hit
CACCTGCACTGCCTCTGGTGGCTCCATCAGCAGTAGCAGTTACTACTGGG16980.14754863326344037No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCTTATCCCTAGACACGTCCAGGAA16880.14667967782608204No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16790.14589761793245956No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16360.14216110955181885No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16280.1414659452019322No Hit
ATAATAGATAGTCCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGTAACTGCTA16260.14129215411446053No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16230.14103146748325304No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15950.1385983922586498No Hit
GCCTCTGGTGGCTCCATCAGCAGTAGCAGTTACTACTGGGGCTGGATCCG15910.1382508100837065No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15740.13677358584019736No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15430.13407982398438661No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14950.12990883788506674No Hit
CAGTATGATAGTTATTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT14530.12625922504816187No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14440.12547716515453938No Hit
ATCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC14280.12408683645476609No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14240.12373925427982277No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14230.12365235873608693No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14180.12321788101740778No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14100.12252271666752114No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13870.12052411916159704No Hit
TCTCTAAGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCACTGGATCTGGGACAGAATTCACT13700.11904689491808791No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT13580.11800414839325796No Hit
GTATTACTGTGCGAGACACACTATGACTACGCCATTCGACCCCTGGGGCC13570.11791725284952212No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13320.11574486425612635No Hit
GAGTGAATTCTGTCCCAGATCCAGTGCCGCTGAACCTTGATGGGACCCCA13170.11444143110008889No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13150.11426764001261722No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13050.11339868457525892No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13050.11339868457525892No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13010.1130511024003156No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12890.11200835587548565No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG12850.11166077370054232No Hit
GGTATCAGCAGAGACCAGGGAAAGCCCCTAAACTCCTGATCTCTAAGGCGTCTACTTTAGAAAGTGGGGTCCCAT12760.11087871380691984No Hit
CTATAATAGATAGTCCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGTAACTGCTA12660.11000975836956152No Hit
GACCAGGGAAAGCCCCTAAACTCCTGATCTCTAAGGCGTCTACTTTAGAA12520.1087932207572599No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12510.10870632521352408No Hit
GTTAGTAACTGGCTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAA12510.10870632521352408No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12440.10809805640737327No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12340.10722910097001495No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTGTTAGT12180.10583877227024166No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12170.10575187672650582No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT12130.1054042945515625No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12050.10470913020167585No Hit
GTCAGAGTGTTAGTAACTGGCTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAACTCCTGATCTCTAAGG11980.10410086139552505No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCTTATCCCTAGACACGTCCAGGAACCACTTCTCCCTGAAGCTGA11900.10340569704563841No Hit
GAGTGTTAGTAACTGGCTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAACTCCTGATCTCTAAGGCGT11750.10210226388960096No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11740.10201536834586512No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG11680.10149399508345013No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11660.10132020399597848No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT451.2697159E-463.64192270
GTGTCGA406.1065293E-754.07031265
TAGGGAT13750.053.63278670
ATTCGTC306.669056E-445.48835818
AATGGTA4750.042.2046470
GTATCAA25400.039.7644541
GTGGTAT2650.037.3412741
TGTCGAG609.408141E-636.3372966
TATCAAC27550.036.1469572
GTACGTA500.002321033735.2966369
ATCAACG28100.035.198253
TCAACGC28150.035.1326754
CAACGCA28600.034.699135
AACGCAG28700.034.698566
CTAGGGA14400.034.62256669
ACGCAGA30700.032.438077
CGAGGGA550.00370302232.0878569
CGCAGAG31200.031.9182248
AGAGTAC31350.031.54656611
CAGAGTA31900.031.11091610
Produced by FastQC (version 0.11.5)