FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295078
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37080.2863148011162262No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34530.2666248673825051No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27730.21411837742591563No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25960.20045124695192104No Hit
CTATTACTGTCAGCAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGA23900.1845448691121307No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTATTCTGGCTCCCAGATACCA23900.1845448691121307No Hit
GTATGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT22820.17620560306020178No Hit
CATCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACCGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA22230.1716498929022036No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGGCTGAAGATT21940.16941064553640783No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21450.16562708964247713No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21070.16269290343902065No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20840.16091694863166542No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20660.15952707095634394No Hit
CTCCTGCAGGGCCAGTGAGATTCTTAGCACCAGGTACTTAGCCTGGTACC20410.1575966852961752No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20240.15628402304726047No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19980.154276421960685No Hit
CAGTAATAGACTGCAAAATCTTCAGCCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19190.1481764032745518No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGGTGGTACCAGGCTAAGTACCTGGTGCTAAGAATCTCA18690.14431563195421432No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18350.1416903074563849No Hit
GTCCCAGACCCACTGCCACTGAACCGGTCTGGGATGCCAGTGGCCCTGCT18020.13914219838496214No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17920.13837004412089465No Hit
GTCTATTACTGTCAGCAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA17600.13589915047587867No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG17500.13512699621181118No Hit
GTAATAGACTGCAAAATCTTCAGCCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17430.13458648822696392No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17270.13335104140445594No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17160.1325016717139817No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTGAGATTCTTAGCAC16650.1285636849672375No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16640.12848646954083076No Hit
CTACCATACTGCTGACAGTAATAGACTGCAAAATCTTCAGCCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC16150.12470291364690003No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15940.1230813896923583No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA15670.12099657317937607No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15530.11991555720968158No Hit
GTGTATTACTGTGCGACAGATTATAGGAGTGCCTGGGTCTGGGGCCAGGG15510.11976112635686809No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15170.11713580185903862No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15100.11659529387419137No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14840.11458769278761588No Hit
GTACTTAGCCTGGTACCACCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCGTCCAGCAGGGCCA14640.11304338425948089No Hit
GATTATAGGAGTGCCTGGGTCTGGGGCCAGGGAACCCTGGTCACCGTCTC14440.1114990757313459No Hit
GGCCAAAAGTGTACGGTGAGCTACCATACTGCTGACAGTAATAGACTGCA14320.1105724906144649No Hit
TCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACCGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT14300.11041805976165142No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGAC14210.10972312092399068No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14190.10956869007117719No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14060.10856488952788945No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13960.10779273526382194No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13880.10717501185256795No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13810.1066345038677207No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13700.10578513417724647No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC13570.10478133363395874No Hit
TCCCTATGCTTGGTAAACCAGACTATCAACAGAAGTTCCAGGGCAGAGTC13360.103159809679417No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13340.10300537882660349No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13250.10231043998894275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC409.551495E-750.6770943
GGTATCA13400.042.1064761
CCTAACG504.4383105E-640.5416762
ATCTCGT1550.039.72015837
CGTATGC1900.038.05380241
GTATCAA35250.036.6135141
GCCGTTA656.4375854E-736.37372215
TCGTATG2000.036.0801140
CGTACTA2100.035.4977229
TGTTAGA14250.035.2181170
CTAGATC1750.035.0068833
ACTAGAT1950.034.87187632
ATGCCGT2150.033.82731244
GACCGTA2200.033.81868726
CCGTTAA701.1491302E-633.776916
GTACTAG2150.033.1308330
ATCAACG40150.032.1438753
TCAACGC40500.031.866094
CAACGCA41100.031.4008925
AACGCAG41350.031.2110426
Produced by FastQC (version 0.11.5)