FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295078
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36680.2832261840599562No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28170.21751585618781263No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27220.21018039067917144No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27100.20925380556229045No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTATTCTGGCTCCCAGATACCA23000.17759548073552328No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGGCTGAAGATT21800.16832962956671335No Hit
GTATGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT21440.16554987421607037No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21350.16485493537840964No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20710.1599131480883777No Hit
CATCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACCGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA20260.15643845390007396No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19990.15435363738709174No Hit
CTATTACTGTCAGCAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGA19890.15358148312302425No Hit
GTCTATTACTGTCAGCAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA19400.14979792722909355No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19370.1495662809498733No Hit
CTCCTGCAGGGCCAGTGAGATTCTTAGCACCAGGTACTTAGCCTGGTACC19090.1474042490104843No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18630.14385233939577385No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18490.14277132342607937No Hit
CAGTAATAGACTGCAAAATCTTCAGCCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18340.1416130920299781No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGGTGGTACCAGGCTAAGTACCTGGTGCTAAGAATCTCA17870.13798396698886092No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG17510.1352042116382179No Hit
GTAATAGACTGCAAAATCTTCAGCCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17300.13358268768367618No Hit
GTCCCAGACCCACTGCCACTGAACCGGTCTGGGATGCCAGTGGCCCTGCT17220.1329649642724222No Hit
GTGTATTACTGTGCGACAGATTATAGGAGTGCCTGGGTCTGGGGCCAGGG16490.12732823814472952No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16390.126556083880662No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA16160.12478012907330678No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16120.12447126736767977No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15960.12323582054517179No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15950.12315860511876504No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15920.12292695883954481No Hit
CTACCATACTGCTGACAGTAATAGACTGCAAAATCTTCAGCCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC15840.1223092354282908No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15520.11983834178327483No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15490.11960669550405457No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG15460.11937504922483434No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTGAGATTCTTAGCAC15430.11914340294561408No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15180.11721301728544536No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14950.11543706247809013No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14810.11435604650839565No Hit
GTACTTAGCCTGGTACCACCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCGTCCAGCAGGGCCA14660.11319781511229439No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14460.1116535065841594No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC14200.10964590549758393No Hit
GGCCAAAAGTGTACGGTGAGCTACCATACTGCTGACAGTAATAGACTGCA13810.1066345038677207No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13780.10640285758850047No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13670.10555348789802621No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGAC13620.10516741076599247No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13530.10447247192833173No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13400.10346867138504398No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13280.10254208626816298No Hit
TCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACCGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT13190.10184714743050226No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13180.10176993200409551No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)