FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134286
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38570.34003769772350184No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34870.3074180585848719No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28470.2509948989937282No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21260.1874306832668304No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21250.18734252207996926No Hit
GCTTAATGTCAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT20900.18425688053982858No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19750.17411834405079493No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19290.1700629294551815No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA18760.16539038655153993No Hit
GGCCAAAAGTGTACGGCTTAATGTCAGTCTGTTGACAGTAGTAAGTTGCA18610.16406796874862248No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18370.1619521002639546No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18320.16151129432964878No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGTCACCTATTTACATTGGTATCAGCAGAAA18000.1586901363500916No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17860.15745587973403533No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17560.1548110441282005No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16240.14317376746252708No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16130.1422039944070543No Hit
AGTCAGAACATTGTCACCTATTTACATTGGTATCAGCAGAAACCAGGGAA16100.14193951084647083No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG16070.14167502728588732No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15860.13982364236180292No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGA15710.1385012245588855No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15590.13744329031655156No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCA15350.13532742183188368No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15200.13400500402896623No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15150.13356419809466044No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC14920.1315364907968537No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14890.13127200723627022No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGTCAC14660.12924429993846348No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14260.125717852464017No Hit
ACATTAAGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA14160.12483624059540538No Hit
CCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATG13890.122455888550154No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13410.11822415158081825No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13330.11751886208592895No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13210.11646092784359502No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTGACATTAAG13170.11610828309615034No Hit
GTCCCAGACCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT13040.11496218766695525No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13040.11496218766695525No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12700.11196470731367573No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC12500.1102014835764525No Hit
ACTTACTACTGTCAACAGACTGACATTAAGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA12470.10993700001586902No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT11670.10288410506697604No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11590.10217881557208677No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11490.10129720370347514No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTA200.0080664750.56223313
ACTAGCG2000.045.6206427
TCGTATG1550.042.25092340
TAGCGCT2200.041.53204329
GACTAGC2200.041.4293726
CTAGCGC2250.040.57319628
CGCTCAT1850.040.3898332
GTATCAA40000.038.865781
CGTATGC1600.038.8516541
CCCACGA2350.038.73364319
GCGCTCA2450.035.9972531
ATCTCGT1550.035.3439437
ACGAGAC2650.034.3608222
TATCAAC45450.033.9767232
ATCAACG46650.033.1749953
ATCTCCG2850.033.11678310
TCAACGC47100.032.856594
CGAGACT2900.032.5644723
CAACGCA47800.032.445965
CCGTCTT1850.032.09707647
Produced by FastQC (version 0.11.5)