FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930189
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41560.4467909209848751No Hit
GATCTATGGTGCATCCGGTTTGCAGACTGGGGTCCCATCAAGATTCAGTGGCAGCGGATTTGGGACCGACTTCA41040.441200659220868No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36210.38927572783595593No Hit
CCATAGATCAGGGCCTTAGGGGCTGTCCCGGGTTTCTGCTGATACCAATT31300.33649075617965807No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAAAACATTAACAA31060.333910635365501No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29080.3126246386487047No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29060.3124096285808583No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25230.27123520058826756No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24630.26478489855287474No Hit
CTTTTGGCCAGGGGACCAAGATGGACATCAAACGAACTGTGGCTGCACCA22870.24586401258238919No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22270.23941371054699637No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21820.23457598402045177No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAAAACATTAACAACTATGTAAATTGGTATCAGCAGAAA21140.22726564171367322No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21010.22586807627267147No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20890.22457801586559292No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20150.21662264335527512No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19660.21135489669303767No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19290.20737721043787874No Hit
ACTTCACTCTCACCATCAGCCGTCTCCAACCTGAAGATTTTGCCACTTAC18520.19909932282579135No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18290.1966267070455574No Hit
ACTTACTACTGTGAACAGACTTACAACTTGAACACTTTTGGCCAGGGGAC18150.19512163657063242No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18100.19458411140101636No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17990.193401556027861No Hit
GACTTACAACTTGAACACTTTTGGCCAGGGGACCAAGATGGACATCAAACGAACTGTGGCTGCACCATCTGTCTT17590.18910135467093248No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17340.18641372882285212No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16990.18265105263553966No Hit
GCCCCTAAGGCCCTGATCTATGGTGCATCCGGTTTGCAGACTGGGGTCCC16680.17931839658392004No Hit
GGCCAAAAGTGTTCAAGTTGTAAGTCTGTTCACAGTAGTAAGTGGCAAAA16630.17878087141430396No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16440.17673827576976292No Hit
CTGCTACTCTGGCTCCGAGGTGCCAAATGTGACATCCAGATGACACAGTC16300.17523320529483793No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15850.17039547876829333No Hit
GATTTGGGACCGACTTCACTCTCACCATCAGCCGTCTCCAACCTGAAGATTTTGCCACTTACTACTGTGAACAGA15120.16254761129189874No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAAATGTGACAT14740.15846242000281663No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCATCTTGGTCC14590.15684984449396844No Hit
ACTGTGAACAGACTTACAACTTGAACACTTTTGGCCAGGGGACCAAGATGGACATCAAACGAACTGTGGCTGCAC14280.15351718844234882No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13970.1501845323907292No Hit
GTAGTAAGTGGCAAAATCTTCAGGTTGGAGACGGCTGATGGTGAGAGTGAAGTCGGTCCCAAATCCGCTGCCA13680.147066886406956No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13560.14577682599987743No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13550.14566932096595422No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13380.1438417353892596No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13180.14169163471079532No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13140.14126161457510247No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13060.14040157430371678No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12580.13524133267540253No Hit
CAGTAGTAAGTGGCAAAATCTTCAGGTTGGAGACGGCTGATGGTGAGAGTGAAGTCGGTCCCAAATCCGCTGCCA12300.13223119172555256No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12210.13126364642024363No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11660.1253508695544669No Hit
GTGCAGCCACAGTTCGTTTGATGTCCATCTTGGTCCCCTGGCCAAAAGTG11570.12438332424915798No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11340.12191070846892405No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11220.1206206480618455No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10950.11771801214591872No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10760.11567541650137768No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAAA10580.11374032589075984No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAAATGTGACATCCAGATGACACAGTCTCCATCCTCC10490.11277278058545093No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10480.1126652755515277No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10440.11223525541583484No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10360.11137521514444915No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10120.10879509433029202No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10120.10879509433029202No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10110.10868758929636879No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10040.10793505405890631No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9970.10718251882144382No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9940.10686000371967419No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9860.10599996344828846No Hit
ACCCGGGACAGCCCCTAAGGCCCTGATCTATGGTGCATCCGGTTTGCAGACTGGGGTCCCATCAAGATTCAGTGG9850.10589245841436525No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9820.10556994331259562No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9800.10535493324474918No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9780.10513992317690275No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9760.10492491310905633No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT9730.1046023980072867No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9660.1038498627698242No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9620.10341984263413134No Hit
AGATGACACAGTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTC9600.10320483256628492No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9560.10277481243059206No Hit
GTCCCGGGTTTCTGCTGATACCAATTTACATAGTTGTTAATGTTTTGACTTGCCCGGCAAGTGATGGTGACT9480.10191477215920636No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9470.10180726712528314No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9340.10040970168428136No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCAC200.006718140652.95535355
ACGGGTT504.2217725E-640.83606316
TACGTAT1004.4712906E-640.48995670
GTATCAA24200.038.39341
GTACGTA1006.149821E-835.0615869
TATCAAC26250.034.8727042
CGTATGC807.032031E-834.53317341
TAGTATG400.002629804234.40927538
CGACTGG1002.1191542E-433.7416370
TCAACGC28000.032.936234
ATCAACG27900.032.932313
TCGTATG851.2128294E-732.45577240
CAACGCA28550.032.1825335
AACGCAG28600.032.1262746
GTGCTAG2250.031.7647971
ATAGTAC553.5292315E-430.936413
TCTTATA1001.6563718E-830.6270482
ACGCAGA30250.030.3706657
CGCAGAG30500.030.344858
GCTCTAC450.00492606930.2700924
Produced by FastQC (version 0.11.5)