FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930189
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42160.4532412230202679No Hit
GATCTATGGTGCATCCGGTTTGCAGACTGGGGTCCCATCAAGATTCAGTGGCAGCGGATTTGGGACCGACTTCA35400.38056782008817563No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30240.32509522258379747No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29700.31928995075194394No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28670.308216932257853No Hit
CCATAGATCAGGGCCTTAGGGGCTGTCCCGGGTTTCTGCTGATACCAATT28600.30746439702039047No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAAAACATTAACAA27860.29950902451007266No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24200.2601621820941766No Hit
CTTTTGGCCAGGGGACCAAGATGGACATCAAACGAACTGTGGCTGCACCA22180.23844616524168744No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22140.2380161451059946No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22080.23737111490245533No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20470.22006280444081794No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20020.21522507791427334No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20010.2151175728803501No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19280.20726970540395553No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAAAACATTAACAACTATGTAAATTGGTATCAGCAGAAA19180.20619465506472343No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18680.20081940336856272No Hit
ACTTCACTCTCACCATCAGCCGTCTCCAACCTGAAGATTTTGCCACTTAC18390.19770175738478957No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17980.1932940509939378No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17870.19211149562078245No Hit
GACTTACAACTTGAACACTTTTGGCCAGGGGACCAAGATGGACATCAAACGAACTGTGGCTGCACCATCTGTCTT17740.19071393017978067No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17660.18985388990839497No Hit
GTAGTAAGTGGCAAAATCTTCAGGTTGGAGACGGCTGATGGTGAGAGTGAAGTCGGTCCCAAATCCGCTGCCA17410.18716626406031464No Hit
GGCCAAAAGTGTTCAAGTTGTAAGTCTGTTCACAGTAGTAAGTGGCAAAA17320.18619871875500568No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17300.1859837086871593No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16820.18082346705884503No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16760.18017843685530574No Hit
ACTTACTACTGTGAACAGACTTACAACTTGAACACTTTTGGCCAGGGGAC16670.17921089154999684No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15960.17157803414144868No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15660.16835288312375227No Hit
CTGCTACTCTGGCTCCGAGGTGCCAAATGTGACATCCAGATGACACAGTC15050.16179507605443624No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14670.15770988476535414No Hit
CAGTAGTAAGTGGCAAAATCTTCAGGTTGGAGACGGCTGATGGTGAGAGTGAAGTCGGTCCCAAATCCGCTGCCA14010.15061455252642206No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCATCTTGGTCC13950.14996952232288277No Hit
ACTGTGAACAGACTTACAACTTGAACACTTTTGGCCAGGGGACCAAGATGGACATCAAACGAACTGTGGCTGCAC13780.14814193674618814No Hit
GAGTGAAGTCGGTCCCAAATCCGCTGCCACTGAATCTTGATGGGACCCCA13780.14814193674618814No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13760.1479269266783417No Hit
GCCCCTAAGGCCCTGATCTATGGTGCATCCGGTTTGCAGACTGGGGTCCC13530.1454543108981078No Hit
GATTTGGGACCGACTTCACTCTCACCATCAGCCGTCTCCAACCTGAAGATTTTGCCACTTACTACTGTGAACAGA13450.1445942706267221No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13340.14341171525356675No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13270.14265918001610425No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12920.1388965038287918No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12710.1366388981164043No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12660.13610137294678823No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAAATGTGACAT12280.13201618165770612No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12270.13190867662378292No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12030.1293285558096258No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11880.12771598030077758No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11830.12717845513116152No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11390.12244823363854014No Hit
GTTCACAGTAGTAAGTGGCAAAATCTTCAGGTTGGAGACGGCTGATGGTGAGAGTGAAGTCGGTCCCAAATCCG11310.12158819336715442No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11210.12051314302792228No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11130.11965310275653658No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10700.11503038629783839No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10630.1142778510603759No Hit
GTGCAGCCACAGTTCGTTTGATGTCCATCTTGGTCCCCTGGCCAAAAGTG10620.11417034602645268No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10440.11223525541583484No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10270.11040766983914023No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10140.10901010439813845No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10000.10750503392321345No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9990.10739752888929024No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAAATGTGACATCCAGATGACACAGTCTCCATCCTCC9920.10664499365182775No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9820.10556994331259562No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9680.10406487283767063No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9550.10266730739666885No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAAA9450.10159225705743671No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9390.10094722685389743No Hit
GTCCCGGGTTTCTGCTGATACCAATTTACATAGTTGTTAATGTTTTGACTTGCCCGGCAAGTGATGGTGACT9370.10073221678605102No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9340.10040970168428136No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9320.10019469161643493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT808.194893E-751.80434870
GTATCAA23450.034.4932861
CTATATG502.0327242E-433.9860341
GTGGTAT3300.032.9561541
ATGGCAA3650.032.1707470
GAGTTTA2050.031.4992521
GTACGTA852.5942356E-531.33034569
AATGGTA4950.030.69887470
GTACGGA450.004974324730.2098061
GTGCTAG2200.029.3515741
ATCAACG27650.029.0979963
TCGGTTT1205.3839845E-428.78019570
GTTATTA1205.3839845E-428.78019570
TATCAAC27850.028.6606542
TCAACGC28050.028.5604934
CAACGCA28450.028.5168915
GATAGGT500.006657927828.4601959
AACGCAG28750.028.3373976
CGCAATG500.006891930528.25958855
ACGTACC2050.028.15020813
Produced by FastQC (version 0.11.5)