FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758701
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAATAGAGCAGACAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCC36390.20691408033542938No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35920.20424165335665356No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC35590.20236526845666203No Hit
GTATTACTGTCTGCTCTATTATGGTGGTGCTTGGGCGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC33620.19116381920519748No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGAA33560.19082265831428993No Hit
GATTTATAGTACAAGCAACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC32550.18507978331734615No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32020.18206619544766278No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGC30840.17535669792648095No Hit
GAGTATTACTGTCTGCTCTATTATGGTGGTGCTTGGGCGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG30810.1751861174810272No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30340.17251369050225138No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACGCCCA30050.1708647461961982No Hit
CAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC29350.16688453580227677No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29070.1652924516447082No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28580.16250630436896324No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28160.16011817813261037No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27860.15841237367807262No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCTA27020.1536361212053669No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26910.1530106595720364No Hit
GTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG26870.15278321897809805No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26560.1510205543750757No Hit
CTATTATGGTGGTGCTTGGGCGTTCGGCGGAGGGACCAAGCTGACCGTCC26100.14840498754478448No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG25920.14738150487206184No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACGCCCAAGCACCACCATAATAGAGCAGACAGTAATACTCAG25170.14311699373571746No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC25000.1421503712114794No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24890.14152490957814887No Hit
GTCCAGGAGTGTTTGTTGCTTGTACTATAAATCAGTGCCCTGGGTACTTG24670.14027398631148785No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23060.13111950240546857No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22170.12605894919033991No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG22160.12600208904185534No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21990.12503546651761727No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT21870.12435314473580217No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20520.11667702469038228No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG20470.11639272394795933No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20160.11463005934493697No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20110.11434575860251402No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19820.11269681429646086No Hit
GTCTAATTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC19640.1116733316237382No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC19440.11053612865404637No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19320.10985380687223126No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19260.10951264598132371No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19140.10883032419950862No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18980.10792056182375515No Hit
CTATAAATCAGTGCCCTGGGTACTTGTCCAGGTTGCTGCTGGAACCAATTTGGATAATAATCACTGGTGACTGCT18950.10774998137830136No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18910.10752254078436302No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTG17970.10217768682681137No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17720.10075618311469658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23350.041.204881
TACGTAT1104.8179845E-640.05744670
CCTAACG603.470086E-739.3598372
GTATCAA59200.037.0665631
CTAACGC656.498685E-736.3311273
CCGAAAC851.4323414E-632.83842568
TATCAAC67200.032.3312952
ATCAACG68650.031.8440743
GTGGTAT7550.031.7469521
TCAACGC69450.031.3783234
AATGGTA7300.031.18627770
AACGCAG70600.030.9645296
CAACGCA70600.030.868965
CGCAGAG73650.029.590618
ACGCAGA74650.029.2846037
AGAGTAC75000.029.0579811
CGATTCA500.006265366428.81797460
GCAGAGT76250.028.581629
CAGAGTA80900.026.98049410
TGGTATC8550.026.4371832
Produced by FastQC (version 0.11.5)