FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879588
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51520.5857287730164578No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44630.5073966447927893No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34860.3963219143508097No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32590.37051437718568236No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29270.33276943296179573No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28340.3221963009954661No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25540.2903632155054412No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23980.27262763930385586No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23800.27058122666521145No Hit
GACTTCACCCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCCGTTTATTACTGTCAGCAATATTATGACCCT23650.2688758827996744No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23490.26705684934310153No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23030.2618271281554546No Hit
AACCAGGACAGCCTCCCAAGTTAATCATTAACTGGGCATCTACCCGGCAA22630.25727954451402246No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20960.23829338281104334No Hit
GTCCCTGACCGATTCAGTGGCAGCGGATCTGGGACAGACTTCACCCTCAC20700.23533745344411247No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20620.23442793671582604No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20300.23078986980268035No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20030.22772025084471367No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC19580.22260421924810253No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT19220.21851139397081362No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18650.21203108728177286No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATCTTAAATACATCATGAC18570.2111215705534864No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGGCCCACTTTGGTCC18370.20884777873277036No Hit
GTGCAGCCACAGTTCGTTTGAGGCCCACTTTGGTCCCTGGGCCGAAAGTG18260.20759719323137651No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17970.20430019509133823No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17830.20270854081683698No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17490.19884309472161968No Hit
CTTAAATACATCATGACCAACAGGAATTACTTAGCTTGGTACCAGCAGAA16500.18758782520907516No Hit
GTCAGGGACCCCGGATTGCCGGGTAGATGCCCAGTTAATGATTAACTTGGGAGGCTGTCCTGGTTTCTGCTGGTA16460.18713306684493194No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16410.18656461888975293No Hit
ATATTATGACCCTCCTTTCACTTTCGGCCCAGGGACCAAAGTGGGCCTCAAACGAACTGTGGCTGCACCAT16220.18440451666007268No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16120.18326762074971464No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15550.17678731406067386No Hit
GAATTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCCAAGTTAA15530.17655993487860225No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAATTCCTGTTGGTCATGATG15450.17565041815031582No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14980.1703070073716331No Hit
GTTTATTACTGTCAGCAATATTATGACCCTCCTTTCACTTTCGGCCCAGGGACCAAAGTGGGCCTCAAACGAA14840.16871535309713184No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14540.16530466536605776No Hit
ATTCAGTGGCAGCGGATCTGGGACAGACTTCACCCTCACCATCAGCAGCC14400.1637130110915565No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14370.1633719423184491No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATCTTAAATACATCATGACCAACAGGAATTA14300.16257611518119847No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13930.15836960031287375No Hit
TCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG13260.15075239771347493No Hit
CTTTGGTCCCTGGGCCGAAAGTGAAAGGAGGGTCATAATATTGCTGACAGTAATAAACGGCCACATCTTCAGC13120.1491607434389737No Hit
GTTAATGATTAACTTGGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA13070.14859229548379468No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13020.14802384752861567No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12730.14472684938857738No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12630.14358995347821935No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12530.1424530575678613No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12480.1418846096126823No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12100.1375644051533218No Hit
GGACCAAAGTGGGCCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12020.13665488842503534No Hit
GTCATAATATTGCTGACAGTAATAAACGGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGGGTGAAGT11960.13597275087882055No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11730.1333578902849971No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11730.1333578902849971No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11660.13256206314774646No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11390.12949244418977976No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11360.12915137541667235No Hit
ATCTTAAATACATCATGACCAACAGGAATTACTTAGCTTGGTACCAGCAG11330.12881030664356494No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11310.12858292746149333No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11300.12846923787045753No Hit
ATTATGACCCTCCTTTCACTTTCGGCCCAGGGACCAAAGTGGGCCTCAAA11300.12846923787045753No Hit
TCCGGGTACATCGTGATGACCCAATCTCCAGACTCCCTGGCTGTGTCTCT11250.1279007899152785No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11070.12585437727663407No Hit
GTAATAAACGGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGGGTGAAGTCTGTCCCAGATCCGCTGCCA10880.12369427504695381No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCCGTTTATTACTGTCAGCAATATTATGACCCTCCTTTCACTTT10870.12358058545591799No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT10860.1234668958648822No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10830.12312582709177479No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10800.12278475831866736No Hit
ATCATTAACTGGGCATCTACCCGGCAATCCGGGGTCCCTGACCGATTCAG10720.12187524159038095No Hit
GATCTGGGACAGACTTCACCCTCACCATCAGCAGCCTGCAGGCTGAAGAT10640.12096572486209453No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10600.1205109664979513No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10500.11937407058759328No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10340.11755503713102045No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10330.11744134753998463No Hit
CATCATGACCAACAGGAATTACTTAGCTTGGTACCAGCAGAAACCAGGAC10310.11721396835791302No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10270.11675920999376982No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10230.11630445162962659No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10050.11425803899098215No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10030.11403065980891054No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10020.11391697021787474No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9920.11278007430751669No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9890.11243900553440929No Hit
GGATCTCTGGTGCCTCCGGGTACATCGTGATGACCCAATCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG9670.10993783453162162No Hit
TTATTACTGTCAGCAATATTATGACCCTCCTTTCACTTTCGGCCCAGGGA9670.10993783453162162No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9620.1093693865764426No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9550.10857355943919197No Hit
GCTTGGTACCAGCAGAAACCAGGACAGCCTCCCAAGTTAATCATTAACTGGGCATCTACCCGGCAATCCGGGGTC9490.10789142189297717No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG9360.10641345720951172No Hit
GGCTGAAGATGTGGCCGTTTATTACTGTCAGCAATATTATGACCCTCCTT9280.1055039404812253No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG9240.1050491821170821No Hit
GTGAAAGGAGGGTCATAATATTGCTGACAGTAATAAACGGCCACATCTTC9070.10311645906947345No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG9060.10300276947843763No Hit
CCTTTCACTTTCGGCCCAGGGACCAAAGTGGGCCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG8950.1017521839770438No Hit
GACCAAAGTGGGCCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT8850.10061528806668578No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8830.10038790888461417No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8810.10016052970254255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)