FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879588
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52900.6014179365793985No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47310.5378654551903845No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38340.43588589203126915No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35900.4081456318185332No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31370.3566442470793144No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30940.3517555946647749No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26940.3062797582504536No Hit
GACTTCACCCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCCGTTTATTACTGTCAGCAATATTATGACCCT25820.2935465240544437No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24900.28308708167914975No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23200.2637598512030632No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22980.2612586802002756No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21680.24647903336562116No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21580.24534213745526315No Hit
GGGTGAAGTCTGTCCCAGATCCGCTGCCACTGAATCGGTCAGGGACCCCG20370.23158569693993097No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20020.22760656125367784No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19760.224650631886747No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19700.22396849434053218No Hit
GTGCAGCCACAGTTCGTTTGAGGCCCACTTTGGTCCCTGGGCCGAAAGTG19590.22271790883913833No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19470.22135363374670866No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19170.21794294601563458No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18880.21464594787559632No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18680.21237215605488025No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGGCCCACTTTGGTCC18350.20862039955069872No Hit
AACCAGGACAGCCTCCCAAGTTAATCATTAACTGGGCATCTACCCGGCAA17940.2039591263182308No Hit
GTCCCTGACCGATTCAGTGGCAGCGGATCTGGGACAGACTTCACCCTCAC17790.20225378245269376No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC16810.19111220253118505No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16370.18610986052560974No Hit
ATATTATGACCCTCCTTTCACTTTCGGCCCAGGGACCAAAGTGGGCCTCAAACGAACTGTGGCTGCACCAT16290.18520034379732328No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16270.1849729646152517No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATCTTAAATACATCATGAC15820.17985693301864053No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC15410.1751956597861726No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15410.1751956597861726No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15250.17337662632959977No Hit
GTAATAAACGGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGGGTGAAGTCTGTCCCAGATCCGCTGCCA15160.17235342001027754No Hit
GTCAGGGACCCCGGATTGCCGGGTAGATGCCCAGTTAATGATTAACTTGGGAGGCTGTCCTGGTTTCTGCTGGTA15150.17223973041924173No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15130.17201235123717012No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14960.1700796281895615No Hit
GTCCCAGATCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTGCCGGGT14860.16894273227920345No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAATTCCTGTTGGTCATGATG14780.16803321555091702No Hit
GTTTATTACTGTCAGCAATATTATGACCCTCCTTTCACTTTCGGCCCAGGGACCAAAGTGGGCCTCAAACGAA14560.16553204454812936No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14500.16484990700191454No Hit
GTCATAATATTGCTGACAGTAATAAACGGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGGGTGAAGT14460.1643951486377713No Hit
CTTTGGTCCCTGGGCCGAAAGTGAAAGGAGGGTCATAATATTGCTGACAGTAATAAACGGCCACATCTTCAGC14440.16416776945569972No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14040.1596201858142676No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13240.15052501853140332No Hit
ATTCAGTGGCAGCGGATCTGGGACAGACTTCACCCTCACCATCAGCAGCC13000.14779646834654409No Hit
GAATTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCCAAGTTAA12630.14358995347821935No Hit
GTTAATGATTAACTTGGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA12620.14347626388718354No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12580.14302150552304033No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATCTTAAATACATCATGACCAACAGGAATTA12460.14165723043061068No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12380.14074771370232428No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12360.14052033452025264No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12340.14029295533818106No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12130.13790547392642918No Hit
GGACCAAAGTGGGCCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12000.13642750924296376No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11820.1343810966043193No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGGGTGAAGTCTGTCCCAGATCCGCTGCCACTGAATCGGTCAGGGACC11800.1341537174222477No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11600.1318799256015316No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11600.1318799256015316No Hit
ATTATGACCCTCCTTTCACTTTCGGCCCAGGGACCAAAGTGGGCCTCAAA11470.1304019609180662No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11460.13028827132703039No Hit
CTTAAATACATCATGACCAACAGGAATTACTTAGCTTGGTACCAGCAGAA11370.12926506500770815No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11330.12881030664356494No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11260.1280144795063143No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11150.1267638940049205No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11080.12596806686766987No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG10930.12426272300213283No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10900.12392165422902542No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10660.12119310404416614No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCCGTTTATTACTGTCAGCAATATTATGACCCTCCTTTCACTTT10600.1205109664979513No Hit
GATCTGGGACAGACTTCACCCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCCGTTTATTACTGTCAGCAAT10550.1199425185427723No Hit
GGCTGAAGATGTGGCCGTTTATTACTGTCAGCAATATTATGACCCTCCTT10360.11778241631309203No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT10340.11755503713102045No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10330.11744134753998463No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10310.11721396835791302No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10240.11641814122066241No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10040.11414434939994633No Hit
TCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG9920.11278007430751669No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9690.11016521371369323No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG9630.10948307616747842No Hit
CAGTAATAAACGGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGGGTGAAGTCTGTCCCAGATCCGCTGCCA9580.10891462821229939No Hit
GTGAAAGGAGGGTCATAATATTGCTGACAGTAATAAACGGCCACATCTTC9530.10834618025712037No Hit
TTATTACTGTCAGCAATATTATGACCCTCCTTTCACTTTCGGCCCAGGGA9500.10800511148401297No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9410.10698190516469075No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9310.1058450092543327No Hit
GGTGAAGTCTGTCCCAGATCCGCTGCCACTGAATCGGTCAGGGACCCCGG9300.10573131966329691No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT9300.10573131966329691No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9230.10493549252604628No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9200.10459442375293888No Hit
GGATCTCTGGTGCCTCCGGGTACATCGTGATGACCCAATCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG9170.10425335497983147No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9170.10425335497983147No Hit
GGTCATAATATTGCTGACAGTAATAAACGGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGGGTGAAGT9120.10368490702465245No Hit
ATCATTAACTGGGCATCTACCCGGCAATCCGGGGTCCCTGACCGATTCAG9100.10345752784258085No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9100.10345752784258085No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT9030.10266170070533022No Hit
GCTGATGGTGAGGGTGAAGTCTGTCCCAGATCCGCTGCCACTGAATCGGT8970.1019795631591154No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8890.10107004643082898No Hit
TCCGGGTACATCGTGATGACCCAATCTCCAGACTCCCTGGCTGTGTCTCT8820.10027421929357835No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)