FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272034
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13080.48082225015990654No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC5950.21872265966754156No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT5890.2165170530154319No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC5870.215781850798062No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC5860.21541424968937706No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA5350.1966665931464449No Hit
CCCCTAGACTTGTCCTCTATGCTAAGAACAAACGGCCCTCTGGGATCCCA5290.19446098649433527No Hit
GCCTTGGGCTGACCAAGGACGGCGACCTTGGTCCCAGTTCCGAAGACAAAGTAGGCACCGCTGGCCCGGGAGCTA4880.17938934103825258No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC4620.16983171221244403No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG4620.16983171221244403No Hit
AGCCAGGACAGGCCCCTAGACTTGTCCTCTATGCTAAGAACAAACGGCCC4230.15549526897373123No Hit
GTCTAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGGTTGCAGAATAGCTT4210.15476006675636134No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC4170.15328966232162156No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC4030.14814324680003235No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG3570.13123359580052493No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAACTGACTCAGGACCCTGTT3480.12792518582236045No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG3470.1275575847136755No Hit
GACTATTACTGTAGCTCCCGGGCCAGCGGTGCCTACTTTGTCTTCGGAACTGGGACCAAGGTCGCCGTCCTTGGT3400.1249843769528809No Hit
GTCCTGGCTTCTGCTGGTACCAGGTTGCAGAATAGCTTCTGAGGCTGTCCCCTTGGCAGGTGATCGTGACTTT3340.12277877030077124No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC3280.12057316364866157No Hit
GACCAAGGACGGCGACCTTGGTCCCAGTTCCGAAGACAAAGTAGGCACCGCTGGCCCGGGAGCTACAGTAATAGT3270.12020556253997663No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAACTGACTCAGGACCCTGTTGTGTCTGTGGCCTTGGGA3200.117632354779182No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT3180.11689715256181213No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT3050.11211833814890786No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT2900.10660432151863371No Hit
CTATTACTGTAGCTCCCGGGCCAGCGGTGCCTACTTTGTCTTCGGAACTG2890.10623672040994876No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCAAGGACGGCGACCTTGGTCCCAGTTCCGAAGACAAAGTAGGCAC2840.10439871486652404No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC301.0683514E-555.9715544
ACAAGCG252.5624898E-455.20468540
TTTACGG200.00803169750.6070628
CCGTTAT200.00813781250.439331
TAGGTAC200.00814373850.43004222
CCTAACG409.87482E-750.4114842
GCCGTTA200.008173414550.3836715
CTAACGC352.6570257E-547.9844473
ACGGGTA452.2259283E-644.8019616
AATGGTA1851.8189894E-1242.1207370
TATAAGA603.5613448E-739.208932
CCGCATT350.001529629138.4158221
CGGGTGC350.001537950238.373446
GTATCAA9300.035.7956541
GTGCTAG502.1598427E-433.626221
CGCATTT400.002942997333.6200322
CTGGTCT400.002942997333.6200322
ACGGGAT400.00295097533.6014716
GGTATCA4050.032.3808061
GCGCGTG2001.1057637E-831.87773370
Produced by FastQC (version 0.11.5)