FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962986
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10350.10747819802157041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGCG2600.037.5719429
GGTATCA25400.036.005571
GTATCAA55150.035.1093751
CCCACGA3150.032.9542519
ACGAGAC3050.031.8505122
GGCGAAT2550.030.53264632
GCGAATC2250.030.12968433
TGCCGCG500.00512081930.03076265
TCTCCGA3350.029.98414411
CGAATCG803.1284599E-629.70731734
ATCAACG66600.029.3156623
TACACCG803.480869E-629.3030955
TATCAAC66500.029.1598242
TCAACGC67600.028.8324584
AACGCAG69500.028.1887936
CAACGCA69600.028.0039425
TCGTATG1005.0445124E-727.5821240
CGGGTAC855.5859873E-627.57508717
ACGGGTA1700.027.57365416
GCCCACG3650.027.52112418
Produced by FastQC (version 0.11.5)