FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283563
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29660.23107552960002742No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28110.21899976861283785No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22720.17700728363157864No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21450.16711295043562333No Hit
GTAATATACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGGTTCAGGGAGA20090.15651744402105702No Hit
GATCTATGCTGCATCCAATTTGGAAAGCGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCA19350.1507522420013665No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19090.1487266304809347No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18650.1452986725232809No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18280.14241607151343566No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18210.14187071456562708No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17610.1371962264415537No Hit
GTATATTACTGTGCGAGAAGGCCGGTAGTACCAACTGCAAAGGATGCTTT17420.13571597186893047No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16730.1303403105262461No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16370.12753561765180205No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16330.12722398511019717No Hit
GAGTTACGGTACCCCCCGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCAT16310.12706816883939473No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16270.12675653629778982No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTG15960.1243413841003519No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15200.11842036580985897No Hit
GTCTCTGGTGGCTCCATTCGTAGTTACTACTGGAACTGGATCCGGCAGTC15120.11779710072664917No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15090.1175633763204455No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14840.11561567293541493No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14780.1151482241230076No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT13940.10860394074930486No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13790.10743531871828653No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGCGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTACCAG13580.10579924787486085No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13440.10470853397924372No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC13320.10377363635442904No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC13290.10353991194822537No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13150.10244919805260824No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12880.10034567839677523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)