FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366767
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT46120.3374386416997191No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA42120.3081724975800557No Hit
GTAATACACAGCCGTGTCTGCGGCGGCCACAGAGGTCAGCTTCAGGGAGA39360.28797885813748797No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC37450.2740042743203487No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGGTGCTGCTTGTAAAAGAGCTGCAGTAATAAT36970.27049233702598907No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG36100.2641269506799623No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG36050.2637611238784665No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36000.2633952970769707No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC34950.255712934245559No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC34920.2554934381646616No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGGTGCTGC30770.22512981364051077No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC30090.22015456914016798No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC29170.21342335599264542No Hit
GATTATTACTGCAGCTCTTTTACAAGCAGCACCACTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT29140.21320385991174792No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT28510.20859444221290094No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCA27660.20237538658747248No Hit
GTGTATTACTGTGCGAGACTTCGAAATGGACTACCTGGGGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC27110.19835129177101876No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG25950.1898641099763164No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCTTTT25800.188766629571829No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGGTGCTGCTTGTAAAAGAGCTG25170.184157211872982No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23880.17471888039439057No Hit
GGTTATACCTATGTCGCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAATAAT23340.170767950938236No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23250.17010946269554356No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATACCTATGTCGCCTGGTACCAACAGCACCCAGGCAAAGC22350.16352458026861932No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGGTG21930.16045163513605465No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21930.16045163513605465No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG21840.15979314689336221No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATACCTATGTCGCCTGGTACCAACAGCA21140.15467157167242113No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG20920.15306193374583962No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC20900.15291560302524132No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG20860.15262294158404469No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC19030.13923368064929867No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18640.1363802315976315No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT18540.13564857799463992No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18060.1321366407002803No Hit
TACCTATGTCGCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAATAATCGGCC17660.12921002628831396No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC17050.1247469393100653No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG16940.12394212034677454No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGCCACAGAGGTCAGCTTCAGGGA16690.12211298633929557No Hit
CTGTTGGTACCAGGCGACATAGGTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA16550.12108867129510736No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16430.12021068697151746No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16330.11947903336852587No Hit
GGTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG16170.11830838760373934No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC15160.11091868621352433No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT15000.10974804044873779No Hit
ACCTACTACAGCCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAG14920.10916271756634452No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTC14880.10887005612514788No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14860.10872372540454955No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14800.10828473324275463No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14720.10769941036036135No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC14350.10499229202929247No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC14100.10316315802181351No Hit
GACATAAGTGGTGCTGCTTGTAAAAGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGT14100.10316315802181351No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13950.10206567761732616No Hit
CTATTATAGTGGGAGAACCTACTACAGCCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAA13760.10067553577164214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT11100.063.27028370
CGTATGC1400.051.74949641
ATCGCGT200.007557839651.4027437
CGATTTA200.00766459251.22070334
GGCGATT450.00968915848.26804770
ATGCCGT1700.044.872444
CTAGCGC2500.044.8031628
TCGTATG1550.044.43620340
TAGCGCT2550.043.95852329
ACTAGCG2650.043.5144527
GGTATCA15600.043.2056541
ACGAGAC2600.042.98539422
GCGCTCA2650.042.38213331
CGCTCAT2100.042.18248432
CTACGGA11300.041.7517169
GACTAGC2700.041.4373126
AGACTAG3000.038.4039925
TCTCCGA2850.038.02224711
GAGACTA2950.037.8937524
GTATCAA39050.036.6886251
Produced by FastQC (version 0.11.5)