FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366767
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT42480.3108064505508254No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA40030.2928809372775316No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC39850.29156396079214675No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG37650.27546758152633183No Hit
GTAATACACAGCCGTGTCTGCGGCGGCCACAGAGGTCAGCTTCAGGGAGA37150.27180931351137394No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC36720.2686632030185101No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGGTGCTGCTTGTAAAAGAGCTGCAGTAATAAT35860.2623709820327825No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC33810.24737208317145495No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG33690.24649409884786505No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC32150.23522663336179467No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30200.22095938810345875No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT29820.2181791044120907No Hit
GTGTATTACTGTGCGAGACTTCGAAATGGACTACCTGGGGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC28190.20625315068332786No Hit
GATTATTACTGCAGCTCTTTTACAAGCAGCACCACTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT28150.20596048924213126No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27720.20281437874926744No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCA26890.1967416538444373No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC26370.19293705510888104No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGGTGCTGC26280.1922785668661886No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGGTGCTGCTTGTAAAAGAGCTG26060.19066892893960713No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG25700.18803497596883742No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCTTTT24460.17896247129174175No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC24370.17830398304904932No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23500.17193859670302253No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATACCTATGTCGCCTGGTACCAACAGCACCCAGGCAAAGC23390.1711337777397318No Hit
GGTTATACCTATGTCGCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAATAAT23330.17069478557793685No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23320.1706216202176377No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG22420.16403673779071343No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGGTG22090.1616222809008412No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21970.16074429657725128No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21650.1584030050476782No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATACCTATGTCGCCTGGTACCAACAGCA20600.15072064221626655No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19180.14033116105378604No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG18930.13850202704630707No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18030.13191714461938284No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC17610.12884419948681816No Hit
TACCTATGTCGCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAATAATCGGCC17530.1282588766044249No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT17260.12628341187634762No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16990.12430794714827034No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16460.12043018305241493No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA15880.11618659215506374No Hit
CTGTTGGTACCAGGCGACATAGGTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA15820.1157475999932688No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG15810.11567443463296964No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGCCACAGAGGTCAGCTTCAGGGA15640.11443062350788392No Hit
ACCTACTACAGCCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAG15570.11391846598578982No Hit
GGTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG15220.11135767837531928No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT14900.10901638684574622No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14810.10835789860305378No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14740.10784574108095968No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC14590.10674826067647229No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC14550.10645559923527566No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14080.10301682730121521No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13770.10074870113194129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)