FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412900
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20110.48704286752240256No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18450.44683942843303465No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15940.38604989101477355No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13750.3330104141438605No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12670.30685395979656094No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12270.29716638411237584No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11450.2773068539597966No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11370.27536933882295955No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10950.2651973843545653No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9990.2419472027125212No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9510.23032211189149915No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8910.2157907483652216No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8830.21385323322838462No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8410.20368127875999031No Hit
GTGTAGAGCTTGCATGCAGTAATAGACCCCAACATCATCAGCCTCCACTG7820.1893921046258174No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTT7700.18648583192056187No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7600.18406393799951562No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7590.18382174860741102No Hit
GTAATAGACCCCAACATCATCAGCCTCCACTGTGCTGATTTTCAGTGTAAAATCTGTGCCTGAGCCACTGCCA7330.1775248244126907No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7310.17704044562848148No Hit
CTATTACTGCATGCAAGCTCTACACACCCCGATCACCTTCGGCCAAGGGA7260.17582949866795833No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7130.17268103657059822No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7080.1714700896100751No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7080.1714700896100751No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6980.16904819568902882No Hit
GCTCTACACACCCCGATCACCTTCGGCCAAGGGACACGACTGGATATTAAACGAACTGTGGCTGCACCAT6970.1688060062969242No Hit
CAGTAATAGACCCCAACATCATCAGCCTCCACTGTGCTGATTTTCAGTGTAAAATCTGTGCCTGAGCCACTGCCA6820.1651731654153548No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6730.16299346088641317No Hit
GGTCTAGTCAGAGCCTCCTTCATAATAATGGCTACACCTATTTGAATTGGTACCTGCAGAAGCCAGGGCAGTCTC6650.16105594574957616No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6290.15233712763380963No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6260.15161055945749577No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6130.14846209736013563No Hit
CTCCACAACTCCTCATCTTTTTGGCTTCTTATCGGGCCTCCGGGGTCCCT6030.14604020343908938No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6000.1453136352627755No Hit
CTACACACCCCGATCACCTTCGGCCAAGGGACACGACTGGATATTAAACG5990.14507144587067086No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG5970.14458706708646163No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5950.14410268830225237No Hit
ATATATTACTGTGCGAGAGAATACACGCAACGATTTTGTAGACCTAACAT5930.1436183095180431No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5870.14216517316541535No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5820.14095422620489223No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5770.1397432792443691No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT5770.1397432792443691No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC5750.13925890046015985No Hit
GTCTATTACTGCATGCAAGCTCTACACACCCCGATCACCTTCGGCCAAGGGACACGACTGGATATTAAACGAA5610.1358682489706951No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5490.13296197626543957No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5470.1324775974812303No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA5350.1295713247759748No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5290.12811818842334707No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG5230.12666505207071932No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATCCCT5160.12496972632598691No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC5140.12448534754177767No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5050.12230564301283604No Hit
AGCCAGGGCAGTCTCCACAACTCCTCATCTTTTTGGCTTCTTATCGGGCC5030.12182126422862678No Hit
GTGGAGGCTGATGATGTTGGGGTCTATTACTGCATGCAAGCTCTACACACCCCGATCACCTTCGGCCAAGGGACA4990.12085250666020828No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA4950.11988374909178978No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA4870.11794623395495277No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC4840.11721966577863889No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG4840.11721966577863889No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT4770.1155243400339065No Hit
GATTTTACACTGAAAATCAGCACAGTGGAGGCTGATGATGTTGGGGTCTATTACTGCATGCAAGCTCTACACACC4750.11503996124969727No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATTCAAATAGGTGTAGCCATTATTATGAAGGAGGCTCTGA4730.11455558246548801No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA4700.11382901428917414No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC4670.11310244611286026No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT4570.110680552191814No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC4530.1097117946233955No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC4440.10753209009445386No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG4360.10559457495761684No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT4350.10535238556551223No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC4280.10365705982077986No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG4260.1031726810365706No Hit
GTAATATATAGCCGTGTCTTCGGCTCTCAGGGCGGTCAATTGTAGATACA4220.10220392346815209No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGCTCAGGCACAGATTTTACACTGAA4220.10220392346815209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGACG2150.072.3763270
ACTAGAG150.002046030271.4860457
TTGCGTT200.006008380654.46555762
TCGACCT200.006508806753.37527555
CAACCGG200.00754319251.4214837
AAGTGCG305.4148276E-447.44468355
GGTATCA5000.043.268451
CTACATT1950.041.9032766
GGTCGGC2300.040.73908260
CGTATGC1450.040.4999441
GTCGGCT2200.039.4521961
TCGGCTA2050.038.96722862
GTAGACC2300.038.8382938
AGACCTA2300.037.46905540
TACATTG2350.036.94624367
TCGTATG1400.036.9337840
CCCACGA1750.036.69647219
GCCGTCT1051.4551915E-1136.52812246
TGTAGAC2450.036.3798237
GGCTACC501.4385644E-436.03841460
Produced by FastQC (version 0.11.5)