FastQCFastQC Report
Wed 8 May 2024
EGAF00004692408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412900
Total Bases30.3 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21520.5211915718091548No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21470.5199806248486316No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16940.41026883022523614No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16680.4039719060305158No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15080.36522160329377573No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14970.36255751998062485No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14380.34826834584645194No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT13300.3221118914991523No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13090.3170259142649552No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12690.30733833858077014No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11140.26979898280455317No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA10980.26592395253087914No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC10700.2591426495519496No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC10550.25550980867038026No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10520.25478324049406637No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10480.25381448292564784No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9860.23879874061516104No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA9800.2373456042625333No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC9700.23492371034148704No Hit
GTGTAGAGCTTGCATGCAGTAATAGACCCCAACATCATCAGCCTCCACTG8970.21724388471784936No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG8880.21506418018890772No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8800.21312666505207073No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA8450.20465003632840884No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8220.19907968031000245No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTT8070.19544683942843305No Hit
CAGTAATAGACCCCAACATCATCAGCCTCCACTGTGCTGATTTTCAGTGT7570.18333736982320176No Hit
GTAATAGACCCCAACATCATCAGCCTCCACTGTGCTGATTTTCAGTGTAA7490.18139985468636474No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC6930.1678372487285057No Hit
CTCCACAACTCCTCATCTTTTTGGCTTCTTATCGGGCCTCCGGGGTCCCT6810.1649309760232502No Hit
CTATTACTGCATGCAAGCTCTACACACCCCGATCACCTTCGGCCAAGGGA6760.16372002906272706No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6680.16178251392589005No Hit
CTACACACCCCGATCACCTTCGGCCAAGGGACACGACTGGATATTAAACG6650.16105594574957616No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT6590.1596028093969484No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6550.1586340518285299No Hit
GGTCTAGTCAGAGCCTCCTTCATAATAATGGCTACACCTATTTGAATTGG6480.15693872608379753No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA6450.15621215790748366No Hit
GCTCTACACACCCCGATCACCTTCGGCCAAGGGACACGACTGGATATTAA6440.15596996851537903No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6410.15524340033906517No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6410.15524340033906517No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6240.1511261806732865No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6220.15064180188907728No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT6180.14967304432065875No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA6040.14628239283119399No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6030.14604020343908938No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC6020.14579801404698473No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC5990.14507144587067086No Hit
GTGGAGGCTGATGATGTTGGGGTCTATTACTGCATGCAAGCTCTACACAC5940.14386049891014774No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC5760.13950108985226448No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATTCAAATAGGTGTAG5640.13659481714700894No Hit
GTCTATTACTGCATGCAAGCTCTACACACCCCGATCACCTTCGGCCAAGG5620.1361104383627997No Hit
ATATATTACTGTGCGAGAGAATACACGCAACGATTTTGTAGACCTAACAT5620.1361104383627997No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC5610.1358682489706951No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATCCCT5580.1351416807943812No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG5470.1324775974812303No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG5430.13150883991281181No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA5390.13054008234439332No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT5360.12981351416807946No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA5280.12787599903124242No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG5220.12642286267861466No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5170.12521191571809157No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT5170.12521191571809157No Hit
GGGTGTGTAGAGCTTGCATGCAGTAATAGACCCCAACATCATCAGCCTCC5160.12496972632598691No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC5110.1237587793654638No Hit
GATTTTACACTGAAAATCAGCACAGTGGAGGCTGATGATGTTGGGGTCTA5100.12351658997335918No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG5090.12327440058125454No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5030.12182126422862678No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5020.12157907483652215No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA4990.12085250666020828No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT4960.1201259384838944No Hit
GTAATATATAGCCGTGTCTTCGGCTCTCAGGGCGGTCAATTGTAGATACA4910.11891499152337129No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT4800.11625090821022038No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT4750.11503996124969727No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG4740.11479777185759263No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA4740.11479777185759263No Hit
GTGTAAAATCTGTGCCTGAGCCACTGCCACTGAACCTGTCAGGGACCCCG4730.11455558246548801No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCT4720.1143133930733834No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT4670.11310244611286026No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGCTCAGGCACAGATTTTACACTGAA4650.11261806732865101No Hit
AGCCAGGGCAGTCTCCACAACTCCTCATCTTTTTGGCTTCTTATCGGGCC4600.11140712036812787No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC4400.10656333252603536No Hit
GGACACGACTGGATATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC4390.10632114313393072No Hit
CCCTGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATG4330.10486800678130297No Hit
GTTCAGTGGCAGTGGCTCAGGCACAGATTTTACACTGAAAATCAGCACAG4300.10414143860498912No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATATCCAGTCGTGTCC4290.10389924921288447No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG4280.10365705982077986No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG4260.1031726810365706No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT4250.10293049164446597No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT4240.10268830225236135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)