FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758775
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGGAGAGCAAGCAGTAATACTCTCCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC81420.4629358502366704No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA53660.30509871927904364No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC53100.301914684936959No Hit
GTATTACTGCTTGCTCTCCTATAGTGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC51070.29037256044690196No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG50960.2897471251297068No Hit
CTCCTATAGTGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC50730.2884393967392077No Hit
CAGTAATACTCTCCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC46930.2668334494179187No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACCACTATAGGAGAGCAAGCAGTAATACTCTC43710.24852525195093172No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGTGTC43330.24636465721880285No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA42340.240735739364046No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40610.23089934755724864No Hit
GTAATACTCTCCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG39080.2222001108726244No Hit
CCACTATAGGAGAGCAAGCAGTAATACTCTCCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTG38410.21839064121334453No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG37550.21350087418800018No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT37530.2133871586757829No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA36030.20485849525948457No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35370.20110588335631335No Hit
GAGTATTACTGCTTGCTCTCCTATAGTGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG34830.19803556452644594No Hit
GCCTGAGGATGAGGGAGAGTATTACTGCTTGCTCTCCTATAGTGGTGCTTGGGTGTTCGGCGGAGGGACCAAG34780.19775127574590268No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC34680.19718269818481612No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG33110.18825603047575729No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC32650.18564057369475911No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC32480.18467399184091202No Hit
CCTATAGTGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA31670.18006851359611092No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT31460.17887450071782915No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG31170.17722562579067816No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCA30530.17358672939972425No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG30510.17347301388750694No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT29300.16659322539835963No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC28960.16466006169066538No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27790.15800770422595273No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27710.15755284217708349No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27350.1555059629571719No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26050.14811445466304674No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25900.1472615883214169No Hit
GCACTGGAGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGCAGAAG25830.1468635840286563No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG25670.14595385993091783No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT25620.14566957115037454No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25000.14214439027163794No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC24980.14203067475942063No Hit
CTCCTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC24540.13952893349063977No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC23780.135207744026382No Hit
GTCCAGGAGTATTTGTTGCTTGTATCATAAATCAGTGTCCTGGGGGCTTG23650.13446859319696947No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTG23620.13429801992864351No Hit
GGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGTGTCCAG23400.1330471492942531No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22410.12741823143949624No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21810.12400676607297693No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACCACTATAGGAGAGCAAGCAGTAATACTCTCC21660.1231538997313471No Hit
GATTTATGATACAAGCAACAAATACTCCTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC21430.12184617134084805No Hit
CCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGTGTCCAGGAGTATTTGTTG21360.12144816704808745No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20720.11780927065713352No Hit
AAGCTGCCCTGACCCTTTCGGGTGCGCAGCCTGAGGATGAGGGAGAGTATTACTGCTTGCTCTCCTATAGTGGTG20350.11570553368111328No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19140.10882574519196599No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC18900.10746115904535827No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGC18510.10524370655712072No Hit
CTCCTATAGTGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT18500.10518684880101206No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18450.10490256002046879No Hit
CCCAAGCACCACTATAGGAGAGCAAGCAGTAATACTCTCCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGG18430.10478884450825147No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18220.10359483162996973No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGC18120.10302625406888317No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG17990.10228710323947066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2400.050.3104241
TCGTATG2650.048.1138340
ACGAGAC3000.047.72673822
ATGCCGT2500.047.0969444
TACGTAT1403.8926373E-1044.9515670
GGTATCA15650.044.666751
ATCTCGT2500.042.5956237
CACGAGA3450.041.50151421
TCTACAT3250.039.9961632
CGTCTTC2700.039.97640248
CCGTCTT2750.039.1994747
GCCGTCT2850.038.98087346
CATCTCG2750.038.684536
TATACAC4100.038.2500843
CCCACGA3850.038.0740519
GTATGCC3300.037.6814342
CTTATAC4100.037.426011
GTATCAA43100.036.9474531
CGAGACC3700.036.85462623
CGAGCCC4100.035.74941615
Produced by FastQC (version 0.11.5)