FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758775
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGGAGAGCAAGCAGTAATACTCTCCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC76470.4347912609628861No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG54400.3093061932310841No Hit
GTATTACTGCTTGCTCTCCTATAGTGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC52970.30117553410754644No Hit
CTCCTATAGTGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC52460.298275788546005No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC51580.2932723060084434No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA51440.2924762974229222No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA46810.2661511563446149No Hit
CAGTAATACTCTCCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC43680.2483546786826058No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACCACTATAGGAGAGCAAGCAGTAATACTCTC40780.2318659294110958No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGTGTC39850.22657815809299084No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT39200.22288240394592826No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37170.21134027945587125No Hit
GCCTGAGGATGAGGGAGAGTATTACTGCTTGCTCTCCTATAGTGGTGCTTGGGTGTTCGGCGGAGGGACCAAG36920.20991883555315488No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC36730.20883853818709047No Hit
CCACTATAGGAGAGCAAGCAGTAATACTCTCCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTG36030.20485849525948457No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG35860.20389191340563748No Hit
GTAATACTCTCCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG35800.2035507668689855No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG35660.20275475828346437No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG35400.20127645662463928No Hit
GAGTATTACTGCTTGCTCTCCTATAGTGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG34860.19820613779477195No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34630.19689840940427283No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC32370.1840485565237168No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT31640.17989794032778497No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31590.1796136515472417No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC31500.1791019317422638No Hit
CCTATAGTGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA31280.17785106110787338No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC29790.16937925544768376No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG29770.16926553993546645No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29740.1690949666671405No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC28920.16443263066623076No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28900.16431891515401342No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCA27930.1588037128114739No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT27130.15425509232278148No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26870.15277679066395644No Hit
CTCCTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC26730.15198078207843527No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26460.1504456226635016No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG25710.14618129095535246No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT25670.14595385993091783No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC25060.14248553680828988No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC24840.14123466617389943No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTG24000.13645861466077241No Hit
GCACTGGAGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGCAGAAG23920.13600375261190317No Hit
GGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGTGTCCAG23230.13208056744040597No Hit
GTCCAGGAGTATTTGTTGCTTGTATCATAAATCAGTGTCCTGGGGGCTTG23010.13082969680601556No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22440.12758880470782222No Hit
GATTTATGATACAAGCAACAAATACTCCTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC21960.12485963241460676No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21770.12377933504854231No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21160.12031101192591434No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20870.11866213699876335No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20820.11837784821822007No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20410.1160466802177652No Hit
CCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGTGTCCAGGAGTATTTGTTG20120.1143978052906142No Hit
CTCCTATAGTGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT19810.1126352148512459No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGC19420.11041776236300833No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACCACTATAGGAGAGCAAGCAGTAATACTCTCC19400.11030404685079104No Hit
AAGCTGCCCTGACCCTTTCGGGTGCGCAGCCTGAGGATGAGGGAGAGTATTACTGCTTGCTCTCCTATAGTGGTG19290.10967861153359582No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGC19170.10899631846029195No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC18520.10530056431322939No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18460.10495941777657744No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18360.1043908402154909No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG18280.10393597816662165No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17950.10205967221503602No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17950.10205967221503602No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATCG200.007765946451.05140710
GGTATCA16000.039.5873531
GTGGTAT5100.038.727671
AATGGTA12400.036.54110370
GTATCAA44100.036.447431
ATGGCAA6900.034.3975670
TATCAAC46150.034.0692062
ATCAACG48000.032.6824343
TCAACGC48450.032.3834844
CAACGCA49400.031.8305265
TGGTATC6150.031.5421642
AACGCAG50200.031.4579726
ACGCAGA52550.029.7273647
CGCAGAG52700.029.7073338
AGAGTAC53650.029.18129311
GAATACG5300.028.897029
CAGAGTA54250.028.85855110
ATACGTA5250.028.5239611
ATAAACG605.8770855E-428.357863
TACGTAT1555.8074525E-527.8408470
Produced by FastQC (version 0.11.5)