FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581077
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23890.4111331200512152No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21640.37241191786974875No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17380.29909977507283886No Hit
GACTTACATTGGCCAGACGTTCGGCCAAGGGACCAAGGTGGATATCAGACGAACTGTGGCTGCACCATCTGTCTT15650.26932747295108905No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15560.2677786248638305No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14550.2503971074401499No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13570.2335318727122223No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13400.23060627076962262No Hit
ATGTAAGTCTGTTGACAGTAGTAAGTTGCAAAGTCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGTAAGTTGTC12870.2214852764779883No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12320.21202009372251868No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12070.20771773792457796No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11640.20031768595211996No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10870.18706643009446253No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10810.18603386470295674No Hit
GTCTCAATCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10180.17519192809214615No Hit
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGGCCAGCTTCAGGGAGA10090.17364308000488748No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9870.16985700690269964No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9850.1695128184388644No Hit
ACTTACTACTGTCAACAGACTTACATTGGCCAGACGTTCGGCCAAGGGAC9780.16830815881544098No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9490.16331742608982974No Hit
CTTACACTCTCACCATCAGCAGTCTGCAACCTGAGGACTTTGCAACTTAC9440.1624569549302416No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCAGCATCACTT9440.1624569549302416No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9440.1624569549302416No Hit
CCTGAGGACTTTGCAACTTACTACTGTCAACAGACTTACATTGGCCAGAC9350.16090810684298293No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATATCCACCTTGGTCC8850.15230339524710151No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8750.15058245292792521No Hit
GATCTATGCTGCAACTAGTCTGCAACGTGGGGTCCCATCAAGGTTCAGTGGCAGTGCATCTGGGACAACTTACA8680.14937779330450182No Hit
GTCTGCAACCTGAGGACTTTGCAACTTACTACTGTCAACAGACTTACATT8540.146968474057655No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8290.1426661182597143No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8020.13801957399793832No Hit
GTCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGTAAGTTGTCCCAGATGCACTGCCACTGAACCTTGATGGGAC7860.13526606628725624No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG7760.13354512396807997No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7660.13182418164890367No Hit
GGACCAAGGTGGATATCAGACGAACTGTGGCTGCACCATCTGTCTTCATC7510.12924276817013924No Hit
GTAGGAGACAGAGTCAGCATCACTTGCCGGGCAAGTCAGAACATTAATAGGTATTTAAATTGGTATCAACAGAGA7480.12872648547438637No Hit
GAGTGTAAGTTGTCCCAGATGCACTGCCACTGAACCTTGATGGGACCCCA7470.12855439124246873No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7450.12821020277863346No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7210.12407994121261037No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7180.12356365851685748No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7130.12270318735726936No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6990.12029386811042254No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT6900.11874502002316388No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGGTGGCCCAGTC6780.11667988924015235No Hit
GTCTATTACTGTGCGATGGGGGAGGAGTACCGCGTTTTATGGAATTGGTT6700.11530313538481131No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6620.11392638152947028No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6570.11306591036988212No Hit
GTAGTAAGTTGCAAAGTCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGTAAGTTGTCCCAGATGCACTGCCA6510.11203334497837637No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6440.11082868535495297No Hit
GTATCAACAGAGACCAGGGAAAGCCCCTAACCTCCTGATCTATGCTGCAA6400.11014030842728244No Hit
GGCCAGACGTTCGGCCAAGGGACCAAGGTGGATATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG6390.10996821419536482No Hit
GTTCAGTGGCAGTGCATCTGGGACAACTTACACTCTCACCATCAGCAGTCTGCAACCTGAGGACTTTGCAACTTA6380.10979611996344718No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6340.10910774303577668No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6280.1080751776442709No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6270.10790308341235327No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT6260.10773098918043564No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6250.107558894948518No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6200.10669842378892988No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6200.10669842378892988No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6150.10583795262934172No Hit
ACTTACACTCTCACCATCAGCAGTCTGCAACCTGAGGACTTTGCAACTTACTACTGTCAACAGACTTACATTGGC6150.10583795262934172No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6100.10497748146975358No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6050.10411701031016543No Hit
ATGCTGCAACTAGTCTGCAACGTGGGGTCCCATCAAGGTTCAGTGGCAGTGCATCTGGGACAACTTACACTCTCA6030.10377282184633019No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5960.10256816222290678No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA5940.10222397375907151No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5940.10222397375907151No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC5940.10222397375907151No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC5930.10205187952715389No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5910.10170769106331863No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGG250.001275556680.5202670
GACGGAA2850.072.98620670
CAGCGTT200.00575592155.06270664
CGACGGA2850.052.98597769
TCGACGG2950.045.56679568
GTGGTAT1850.043.8542021
TGTCGAC2950.042.85695366
GTCGACG3000.042.811667
CGCGGAC2950.041.3229958
CGTGTCG2950.041.06371364
TTAACGG759.567884E-1041.0028435
GTGTCGA3050.039.94890665
TCGCGGA3100.039.2196457
CGTATGC1150.038.99230641
GACGTGT3100.038.7579162
CGGACGT3100.038.408960
CGTGTCT3000.038.29503313
ACGTGTC3150.038.284763
CCGTGTC2950.037.79868712
TCGTATG1200.037.30209440
Produced by FastQC (version 0.11.5)