FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391155
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57060.410162778410745No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT23960.17223098792010955No Hit
GTATTACTGTCAGCATTATGGTGCCTCCCCTCGAACGTTCGGCCAAGGGA23850.17144027804234613No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21140.15196006196290132No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21120.1518162965305807No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20770.14930040146496976No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20160.14491555577919069No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18350.13190478415417403No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17860.12838253106231873No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16840.12105049401396682No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15900.1142935186948974No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15030.10803972238895018No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT14830.10660206806574393No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATTGTGAGAGTGAAGTCTCTCCCAGACCCACTGCCA14630.10516441374253767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23650.043.19031
CGTATCG405.7424397E-542.1284510
GTATCAA63950.038.8509561
TACGAAC400.002847768133.8561328
CGATCAG502.1357005E-433.7027610
CCTAACG1004.9112714E-1033.697912
CTAACGC906.531991E-933.697913
TATCAAC76650.032.313072
ATCAACG77050.032.101583
TCAACGC77550.032.0707244
CAACGCA79150.031.5087095
AACGCAG80700.031.1134516
CCGTATC652.7241114E-531.1102379
CGCAGAG84650.029.6217968
ACGCAGA84600.029.5994687
GTGGTAT8750.029.280621
AGAGTAC86550.028.9325811
CAGAGTA89000.028.17399210
GCAGAGT90450.027.6850749
TGGTATC9450.027.1009652
Produced by FastQC (version 0.11.5)