FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708171
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGAGCCGCTCCAACATCGGGGCAGGTTTTGATGTACATTGGTACCAGCAACTTCCGGGAAAAGC52010.3044777132968538No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT49710.29101301918836No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA47250.27661165070710136No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37640.22035264619291628No Hit
GTAATAGAGACCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATACA37240.2180109602610043No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG37050.2168986594433461No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC36960.21637178010866592No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC33840.1981066298397526No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC32220.18862280181550908No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCGACCACCCAGGC31950.18704216381146851No Hit
GATTATTACTGCCAGTCCTTCGACAGTAGCCTGGGTGGTCGGGTCTTCGG31250.1829442134306226No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC30700.1797243952742436No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCGACCACCCAGG29820.1745726862240373No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC29040.17000639865680894No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCGACCACCCAGGCTACTGTCGAAGGA26460.15490252439597674No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26380.15443418720959434No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG24830.14536015422343548No Hit
CAGTAATAGAGACCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATA24680.1444820219989685No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23850.13962302369025115No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23530.1377496749447216No Hit
GGATGAGACTGATTATTACTGCCAGTCCTTCGACAGTAGCCTGGGTGGTCGGGTCTTCGGAACTGGGACCAAGGT22540.13195400226323944No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22390.13107587003877247No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGACTGATTATTACTGCCAGT22380.13101732789047468No Hit
CTATTACTGCGCGAGAGACGGGCCAGATATTGTAGTAGTGCCAGATGCCC22310.13060753285239007No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG22290.13049044855579447No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22240.1301977378143055No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC22090.1293196055898385No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG21960.1285585576619671No Hit
CTCTATTACTGCGCGAGAGACGGGCCAGATATTGTAGTAGTGCCAGATGCCCGGGGATACCACCACTCTTACGG21670.12686083536133091No Hit
GTCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC21550.12615832958175732No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21360.12504602876409915No Hit
GTAATAATCAGTCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG21050.12323122216686735No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC20560.1203626569002752No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC20320.11895764534112802No Hit
CTCCAACATCGGGGCAGGTTTTGATGTACATTGGTACCAGCAACTTCCGGGAAAAGCCCCCAAACTCCTCATCT19980.11696721229900287No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19950.11679158585410947No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19780.11579636933304686No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGGCAGAGAATCGGTC19560.11450844207049529No Hit
ACAGTAGCCTGGGTGGTCGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA18980.11111299746922293No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18890.11058611813454272No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18720.10959090161348016No Hit
GTGCAGGAGATGGTGACCGTCTGCCCTGGGGCCCCAGACACTGAGGGCGG18450.10801026360943958No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18350.10742484212646157No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18210.10660525205029238No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG18130.10613691486390998No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17820.10432210826667823No Hit
GGCTACTGTCGAAGGACTGGCAGTAATAATCAGTCTCATCCTCAGCCTGG17680.10350251819050904No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17450.10215604877965964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT453.0873707E-853.53806739
TACGGAC200.00777120751.04262
TCGACGT2500.050.4743329
TCGTATG1250.049.6359140
TGTCGTA352.3191767E-549.11961438
ACTCGAC2800.045.02013427
GGTATCA17400.044.98271
GACTCGA3000.043.13926326
CTCGACG2950.042.75353228
AGACTCG3050.042.4183925
TCTCGTA1150.041.8584538
ACGAGAC3050.041.2815622
CGACGTC3000.040.95998830
CACGAGA3100.040.61572621
ATCTCCG3250.039.78122710
ATGCCGT1400.039.6463644
CGTATGC1400.039.4661441
CGAGACT3300.039.19109723
GTCGTAC350.001441935838.8918761
ACGTCAT2850.038.3927832
Produced by FastQC (version 0.11.5)