FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708171
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGAGCCGCTCCAACATCGGGGCAGGTTTTGATGTACATTGGTACCAGCAACTTCCGGGAAAAGC53010.3103319281266337No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT43960.25735128391712536No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC39860.23334900311502768No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA37790.22123077841738328No Hit
GTAATAGAGACCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATACA35480.20770754216059167No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC34000.19904330421251737No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCACCATCTCCTG33580.19658453398400977No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32720.19154990923039905No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC32230.1886813439638069No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG32160.18827154892572232No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30890.1808366960919018No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC30480.17843646801169202No Hit
GATTATTACTGCCAGTCCTTCGACAGTAGCCTGGGTGGTCGGGTCTTCGG29080.17024056725000014No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC26770.15671733099320853No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG25350.14840434593492102No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCGACCACCCAGGC24620.1441307691091817No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23920.14003281872833573No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCGACCACCCAGGCTACTGTCGAAGGA23680.13862780716918857No Hit
GGATGAGACTGATTATTACTGCCAGTCCTTCGACAGTAGCCTGGGTGGTCGGGTCTTCGGAACTGGGACCAAGGT23340.1366373741270634No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23270.13622757908897878No Hit
CTCTATTACTGCGCGAGAGACGGGCCAGATATTGTAGTAGTGCCAGATGCCCGGGGATACCACCACTCTTACGG22510.13177837581834606No Hit
CAGTAATAGAGACCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATA22450.13142712292855926No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGACTGATTATTACTGCCAGT22370.13095878574217687No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22280.13043190640749666No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT22190.12990502707281648No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG22070.1292025212932429No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22050.1290854369966473No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21750.1273291725477133No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCGACCACCCAGG21220.12422643868792996No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCAC20990.12287996927708059No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG20560.1203626569002752No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC20460.11977723541729722No Hit
CTATTACTGCGCGAGAGACGGGCCAGATATTGTAGTAGTGCCAGATGCCC20240.11848930815474563No Hit
CTCCAACATCGGGGCAGGTTTTGATGTACATTGGTACCAGCAACTTCCGGGAAAAGCCCCCAAACTCCTCATCT20160.11802097096836324No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19860.11626470651942926No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCAC19270.11281071976985911No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19000.11123008176581851No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18710.10953235946518235No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC18640.10912256442709777No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18070.10578566197412319No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18000.1053758669360386No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17900.10479044545306061No Hit
CTCCTGTGTGGTCTCTGGATTCCCCTTCAGTGACTACTACATAAGTTGGGTTCGCCAGGCTCCAGGGAAGGGG17890.10473190330476281No Hit
ACAGTAGCCTGGGTGGTCGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA17560.10280001241093543No Hit
GTGCAGGAGATGGTGACCGTCTGCCCTGGGGCCCCAGACACTGAGGGCGG17490.10239021737285085No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17420.10198042233476624No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17240.10092666366540587No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT17090.10004853144093886No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT100.007164176144.5002170
GGTATCA18650.042.848631
GGTACTA503.04071E-642.83551860
GTATCAA44250.036.733521
TATCAAC51850.031.0714362
TCTATAC553.5558458E-430.8986193
TCTGGAG17850.030.76195370
ATCAACG52350.030.6448173
AACGCAG53000.030.1469066
TCAACGC53300.029.974594
AATGGTA6550.029.78248870
CAACGCA54350.029.3980875
GTACTAT754.7988757E-528.63361461
ACGCAGA56000.028.5318957
CGCAGAG56250.028.3446488
AGAGTAC56450.028.18317611
CAGAGTA56900.028.02085110
GCAGAGT57150.027.8982759
TATCTAT805.8173173E-527.83616867
CGGTACT657.225057E-427.37364859
Produced by FastQC (version 0.11.5)