FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671391
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC41450.617374972259086No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30010.4469824588056736No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC28690.42732178417643374No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24660.36729714875534525No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23030.34301919447832935No Hit
CCCCTGTGCTGGTCATCTATGAGGACAGTAAACGACCCTCCGGGATCCCT20660.3077193468485577No Hit
GTAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGG19840.29550589745766626No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19400.2889523392479196No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATGATTACTA19280.28716500519071597No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTT18800.2800156689619015No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18440.27465366679029063No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG18320.272866332733087No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17540.2612486613612634No Hit
CTACTACTGTTACTCAACAGACAGCAGTAGTAATCATGGGGTGTTCGGCG16590.2470989334084014No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15760.23473653951274295No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15700.23384287248414112No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC15680.2335449834746072No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG14640.2180547549788424No Hit
CCCCATGATTACTACTGCTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCA14540.21656530993117273No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG14440.21507586488350305No Hit
TCTATGAGGACAGTAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC14390.21433114235966821No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14290.21284169731199853No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13920.20733075063562068No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13820.205841305587951No Hit
GACTACTACTGTTACTCAACAGACAGCAGTAGTAATCATGGGGTGTTCGG13450.20033035891157316No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13060.19452152322566135No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA12740.18975529907311836No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12690.18901057654928352No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12510.18632957546347806No Hit
CAGTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGT12420.18498907492057534No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12340.1837975188824396No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12310.18335068536813867No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12120.18052073977756627No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12030.17918023923466356No Hit
GTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGG11970.17828657220606176No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAGTAAACGACCCTCCGG11950.1779886831965278No Hit
ATCATGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC11930.17769079418699388No Hit
GTGCTGGTCATCTATGAGGACAGTAAACGACCCTCCGGGATCCCTGAGAG11790.1756055711202563No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11560.17217984751061602No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATGATTACTAC11450.17054145795817935No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11250.16756256786284No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10780.16056217613879245No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC10380.15460439594811368No Hit
GCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAG10290.15326389540521096No Hit
GATTACTACTGCTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCATCCTCC10210.1520723393670752No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9820.14626350368116345No Hit
GTAGTAATCATGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA9820.14626350368116345No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9800.1459656146716295No Hit
CTACTGCTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCATCCTCCACCTG9650.14373144710012495No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9580.14268883556675618No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC9570.14253989106198922No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGGCCAGGCCC9530.14194411304292134No Hit
GTCGTTTACTGTCCTCATAGATGACCAGCACAGGGGCCTGGCCTGACTTC9180.13673105537607744No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9140.13613527735700956No Hit
GCTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGTTACTCAA8860.13196483122353442No Hit
CTGATAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTTA8840.1316669422140005No Hit
GACCAGCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATT8790.13092221969016565No Hit
GACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGTTACT8740.13017749716633079No Hit
GGATGAAGCTGACTACTACTGTTACTCAACAGACAGCAGTAGTAATCATGGGGTGTTCGGCGGAGGGACCAAG8730.13002855266156382No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8480.12630494004238962No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8390.12496443949948689No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT8290.1234749944518172No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC8280.12332604994705022No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8030.11960243732787601No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA7790.11602776921346876No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7760.11558093569916784No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7680.11438937966103209No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATGATTA7680.11438937966103209No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7580.11289993461336241No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA7520.1120062675847606No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7390.11006998902279No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG7380.10992104451802304No Hit
GGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC7330.1091763219941882No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC7270.10828265496558637No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC7250.10798476595605244No Hit
GGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGG7210.10738898793698455No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC7130.1061974318988488No Hit
GCTTGGTCCCTCCGCCGAACACCCCATGATTACTACTGCTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCATCCT7120.10604848739408183No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7110.10589954288931487No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGG7010.10441009784164519No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC6990.10411220883211124No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC6940.1033674863082764No Hit
AGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAGTAAACGACCC6920.10306959729874246No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTG6900.10277170828920852No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG6890.10262276378444155No Hit
CCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGT6850.10202698576537367No Hit
CAGCAGTAGTAATCATGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC6790.10113331873677187No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG306.72114E-445.413834
GTGGTAT1700.044.0879821
CGTATGC702.1855158E-839.4313341
GTATCAA13150.036.7882421
TCGTATG852.9976945E-936.4879240
TAGGCAA1608.0428435E-833.99706370
GGTATCA5350.033.7495961
CGGTTCA1055.0931703E-1033.57124355
TATCAAC14500.033.3556752
ACGAGAC959.955329E-932.2677222
ATCAACG15000.032.016753
AATGGTA4050.031.89847870
GCGTCTC450.004071059631.4714557
TCAACGC15300.031.3889714
ACCCTAG450.004459931530.89024246
CAACGCA15700.030.589255
ATGCCGT802.6745656E-630.3085344
CACCCGC450.004918703330.27814124
AACGCAG16050.030.1344096
GAGCAAA1603.0879128E-629.74742970
Produced by FastQC (version 0.11.5)