FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059497
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCATTTAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA44900.4237860041132726No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42870.4046259687379955No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36310.34270979530852846No Hit
GTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAATT30550.2883443747363136No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29980.28296446332552144No Hit
GAGTTACAGTACCCTGTACCCTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCAT26110.24643769637856452No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25380.23954763439632204No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25260.23841502146773422No Hit
ACAGTACCCTGTACCCTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGA24660.23275195682479513No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23910.22567312602112133No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT23440.22123705871748572No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22940.21651783818170323No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22230.209816545020892No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22180.20934462296731374No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20530.19377119519923133No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19960.18839128378843922No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19280.18197314385977498No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT18810.17753707655613937No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18740.17687638568112982No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18680.17631007921683592No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18470.17432800659180725No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18390.17357293130608203No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17970.1696087860560247No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACAA17960.16951440164530904No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17060.16101980468090046No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC16910.1596040385201657No Hit
GTACAGGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAG16850.1590377320558718No Hit
GTTACAGTACCCTGTACCCTTTTGGCCAGGGGACCAAGCTGGAGCTCAAA16700.15762196589513702No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC16690.1575275814844214No Hit
ACCCTGTACCCTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC16680.15743319707370573No Hit
GACCAGGGAAAGCCCCTAAGCTCCTGATCTATACTGCATTTAATTTGCAA15740.14856106246643455No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCCCCTGGCCAAAAGGGTACAGGGTA15460.14591829896639633No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15360.1449744548592398No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15230.14374745751993634No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCCCCTGGCCAAAAGGG14890.14053838755560422No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14760.13931139021630076No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14090.13298763469835215No Hit
GTAGGAGACACAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACAACTATTTAAATTGGTATCAGCAGAGA14080.1328932502876365No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC14020.1323269438233426No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13940.13157186853761738No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13940.13157186853761738No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13780.13006171796616697No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13460.12704141682326614No Hit
GGCCAAAAGGGTACAGGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCA13310.12562565066253137No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13250.12505934419823747No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA13090.12354919362678705No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13090.12354919362678705No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCTGTACCCTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAA12950.12222781187676793No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT12240.11552651871595673No Hit
ATACTGCATTTAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA12160.11477144343023152No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11890.11222306434090894No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11780.11118483582303679No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCA11350.1071263061622638No Hit
GTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCGGGCAAGTCAGA11300.10665438410868554No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG11290.10655999969796989No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA11210.10580492441224468No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10920.1030677765014908No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10630.10033062859073692No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10610.10014185976930563No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACT200.00681998652.7547951
GACGCGT2750.044.3807826
ACGCGTA2850.044.04431527
GCGTAGT2900.043.33410629
TCGTATG2550.043.1864140
CGTATGC2650.042.9604741
CGCGTAG2950.042.5814928
ACGGGTT405.5809694E-542.3314816
AGACGCG3150.040.89183825
ACGAGAC3250.040.65548722
CGAGACG3200.040.23769423
AATCTCG2050.040.0420236
CGTAGTA3200.039.31992330
ATGCCGT2850.038.9581244
GAGACGC3350.038.44145224
ACCGTAT451.1175404E-437.6262058
ATCTCGT2150.036.62719737
GCCGTCT2400.036.24795546
GTATGCC3650.035.0351542
GTATCAA33950.034.9273951
Produced by FastQC (version 0.11.5)