FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1508959
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATTCAGGATCCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT86410.57264644036054No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCTAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC81100.5374566174428861No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51670.34242149720436404No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGCAAAGCCCCTAAGGTCCTGATCTATAAGGCGT48810.32346803326001566No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46440.30776184111032834No Hit
AGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC40920.27118033028067695No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38160.25288957486585123No Hit
CTCTAAACTAGACGCCTTATAGATCAGGACCTTAGGGGCTTTGCCTGGTT35570.23572542395121407No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34380.22783919244989426No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34020.22545344174361265No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC33280.2205493986251449No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29600.19616172473871063No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC28620.1896671811493884No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28310.18761278470786813No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28120.18635363850177505No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA28080.186088555089966No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTAGCAACTTA27320.1810519702655937No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26490.17555148947055552No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA26290.1742260724115102No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25040.16594221579247678No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24570.16282748570372027No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23940.15865242196772741No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23480.15560396273192312No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23210.15381464970221193No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT23170.15354956629040287No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23110.15315194117268924No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22620.14990466937802815No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA21710.14387402175937186No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGCAAAGCCCCT21440.14208470872966064No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21040.13943387461156995No Hit
ACTTATTACTGCCAACAGTATAATAGTTATTCAGGATCCACCTTCGGCCA20570.1363191445228134No Hit
CAGTATAATAGTTATTCAGGATCCACCTTCGGCCAAGGGACACGACTGGA19910.13194526822796376No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19840.13148137225729792No Hit
GTCCTGATCTATAAGGCGTCTAGTTTAGAGAGTGGGGTCCCATCAAGGTT19320.12803528790378002No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19250.12757139193311415No Hit
CCTTAGGGGCTTTGCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA19230.1274388502272096No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT19190.12717376681540055No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC19100.12657732913883016No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT18300.12127566090264878No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18210.12067922322607837No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC17930.1188236393434149No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG17640.11690178460779915No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTTTCCTTCCACC17230.11418467963675619No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17190.11391959622494713No Hit
GATCTATAAGGCGTCTAGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA16820.11146757466571325No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16790.11126876210685645No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16680.1105397827243815No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16540.10961199078304976No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16430.10888301140057483No Hit
ATTATACTGTTGGCAGTAATAAGTTGCTAAATCATCAGGCTGCAGGCTGC16430.10888301140057483No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15770.1045091351057252No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15170.10053288392858918No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTG200.007667669651.2157634
CGATACA200.00795338350.74397712
AATCGCG606.035407E-945.62430236
CTAACGC405.6116118E-542.2936553
CCCACGA3350.041.4126519
CGAGACG3300.041.03106723
TGAATCG1200.039.83448434
GACGCTC3500.038.71981426
ACGAGAC3500.038.6774622
TCTCCGA3500.038.6620811
TCGTATG1250.038.58174540
GAGACGC3600.037.6217924
CCTAACG451.1235694E-437.5931172
ATCGCGT557.750434E-637.3803437
ATCTCCG3700.036.57223510
CGCTCAT3850.036.1325828
CACGAGA3800.035.62161321
ATCTCGT1350.035.53439737
GTATCAA43200.035.248221
AGACGCT3850.035.18698525
Produced by FastQC (version 0.11.5)