FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1508959
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGTTGGCAGTAATAAGTTGCTAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC83520.5534941638573347No Hit
GTATAATAGTTATTCAGGATCCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT82500.5467345368562035No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55680.3689961092382232No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGCAAAGCCCCTAAGGTCCTGATCTATAAGGCGT44680.2960981709907294No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37340.24745536492376533No Hit
AGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC37180.24639503127652906No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37160.24626248957062452No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35220.2334059440978847No Hit
CTCTAAACTAGACGCCTTATAGATCAGGACCTTAGGGGCTTTGCCTGGTT34090.2259173377142785No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32690.21663941830096112No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31200.20676506121107335No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31150.206433706946312No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC29390.19477003682671298No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC28690.19013107712005428No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27500.1822448456187345No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA25200.16700254943971307No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25040.16594221579247678No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTAGCAACTTA24930.16521323641000185No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24870.16481561129228828No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24420.16183342290943623No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA24290.16097190182105678No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23520.1558690461437322No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23010.15248923264316658No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT22800.151097544731169No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22510.14917568999555322No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22260.14751891867174655No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21290.14109064593537662No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21290.14109064593537662No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21190.14042793740585396No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21080.13969895802337903No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC19940.13214408078682058No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19780.13108374713958432No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA19040.12617970402111653No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA18960.1256495371974984No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18890.12518564122683254No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT18550.12293243222645546No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC18540.12286616137350317No Hit
GTCCTGATCTATAAGGCGTCTAGTTTAGAGAGTGGGGTCCCATCAAGGTT18500.12260107796169412No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18500.12260107796169412No Hit
CAGTATAATAGTTATTCAGGATCCACCTTCGGCCAAGGGACACGACTGGA17980.11915499360817623No Hit
CCTTAGGGGCTTTGCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA17820.11809465996093997No Hit
ACTTATTACTGCCAACAGTATAATAGTTATTCAGGATCCACCTTCGGCCA17620.11676924290189461No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT17550.11630534693122874No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG17410.115377554989897No Hit
ATTATACTGTTGGCAGTAATAAGTTGCTAAATCATCAGGCTGCAGGCTGC17410.115377554989897No Hit
GTAATAAGTTGCTAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG17120.11345570025428126No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGCAAAGCCCCT17100.11332315854837673No Hit
GATCTATAAGGCGTCTAGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA16660.11040724101847699No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16290.1079552194592431No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16260.10775640690038629No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTTTCCTTCCACC16150.10702742751791136No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15620.10351507231144119No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15540.10298490548782306No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15420.10218965525239587No Hit
GTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA15150.10040034222268464No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAC200.0080058650.6596216
TAACGGC853.2378011E-936.21001436
GTATCAA47950.035.641941
CGCATAA1201.3176123E-534.59211370
CTAACGC601.5592537E-533.755173
TATCAAC52200.032.0145232
ATCAACG52600.031.8941443
TCAACGC53250.031.6284624
AACGCAG54800.031.2296946
CAACGCA54200.031.2006995
TTAACGG1001.5967089E-830.74547835
GTGGTAT5950.030.6532331
CGCAGAG58250.029.5538858
AATGGTA12200.029.4883670
CCTAACG704.4650755E-528.9377982
AGAGTAC59400.028.86805511
TTCCGCG500.0064935228.60744957
GCAGAGT60550.028.5985189
GGTCTAT500.00857975527.0202561
ACGCAGA63600.027.014757
Produced by FastQC (version 0.11.5)