FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597960
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18670.3122282426918188No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC16820.2812897183758111No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14360.24014984279884943No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCCCCAGATGGTTACCACTGCTGTCC14160.23680513746738913No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13070.2185764934109305No Hit
CCCCTGTACTTGTCATCTATGGTGAAAACAACCGGCCCTCAGGGATCCCA13030.21790755234463843No Hit
GTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGGTT12280.20536490735166232No Hit
AGCCAGGACAGGCCCCTGTACTTGTCATCTATGGTGAAAACAACCGGCCC12200.2040270252190782No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12170.20352531941935917No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCCCCAGATGG11830.19783932035587665No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11580.19365843869155128No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC11040.18462773429660848No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10500.17559702990166567No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10390.17375744196936252No Hit
CAGTAATACAGAGCCGTGTCCTCAGGTCTCAGGCTGCTCATTTGTAGATA10060.168238678172453No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG9980.16690079603986888No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9570.1600441501103753No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9320.1558632684460499No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9310.1556960331794769No Hit
GTAATACAGAGCCGTGTCCTCAGGTCTCAGGCTGCTCATTTGTAGATACA9290.15536156264633086No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA9120.1525185631145896No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGGTAACCATCTGGGGGTGGTATTCGGCGGAGGGACCAAGCTGACCGT9060.15151515151515152No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8950.14967556358284836No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8860.1481704461836912No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG8740.14616362298481503No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG8300.13880527125560238No Hit
CTCCTCACTCTTTGCATAGATTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA8190.13696568332329923No Hit
GACTATTACTGTAACTCCCGGGACAGCAGTGGTAACCATCTGGGGGTGGT8090.13529333065756907No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7870.13161415479296276No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC7860.13144691952638973No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAC7640.1277677436617834No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7590.12693156732891833No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGCTCAAGGA7550.12626262626262627No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7320.12241621513144692No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7250.12124556826543582No Hit
CTCTTTGCATAGATTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT7240.1210783329988628No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGCTCAAGGAAG7210.12057662719914376No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT7110.11890427453341362No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG7100.1187370392668406No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC6750.11288380493678508No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC6730.11254933440363905No Hit
GTATTACTGTGCGAAAGAGCATAGCAGCAGCCCCGGGTGGTTCTTTGACTCCTGGGGCCAGGGAACTCTGGTCGC6710.11221486387049302No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6700.11204762860392No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA6610.11054251120476287No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT6590.11020804067161684No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6420.10736504113987558No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGATTCTGTGGTTTCTTCTGAGCTGACTCAGGA6350.10619439427386448No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGGTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT6260.10468927687470733No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAACCTATTATGCAAGCTGGTA6250.10452204160813433No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG6230.1041875710749883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATC800.051.40618533
TAACGCT200.007651992651.23854
TTATACA2400.048.391922
CGAGACA2400.048.3919223
ACGAGAC2400.048.38382322
ATGCCGT1300.047.96707544
TATACAC2450.047.4043243
GTATGCC1250.046.94707542
CGTATGC1250.046.90742541
GAGCGAT2200.046.72113432
TGGTATC1400.046.358642
TGCCGTC1500.046.24543445
TCGTATG1200.045.9449940
TCTCGTA751.8189894E-1145.83669738
CTTATAC2550.045.5491451
GGTATCA6000.044.979781
TACACAT2600.043.3556565
TGAGCGA2450.043.3339531
CACGAGA2750.042.2258821
AGGGGGG1803.6379788E-1241.6662570
Produced by FastQC (version 0.11.5)