FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597960
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19000.31774700648872833No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC17030.2848016589738444No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16900.28262760050839525No Hit
CCCCTGTACTTGTCATCTATGGTGAAAACAACCGGCCCTCAGGGATCCCA13790.23061743260418757No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13460.2250986688072781No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13260.2217539634758178No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCCCCAGATGGTTACCACTGCTGTCC12350.20653555421767342No Hit
GTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGGTT11400.190648203893237No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11220.18763796909492272No Hit
AGCCAGGACAGGCCCCTGTACTTGTCATCTATGGTGAAAACAACCGGCCC10950.18312261689745135No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCCCCAGATGG10720.17927620576627198No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG10210.17074720717104824No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC9980.16690079603986888No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC9960.16656632550672285No Hit
GTAATACAGAGCCGTGTCCTCAGGTCTCAGGCTGCTCATTTGTAGATACA9580.1602113853769483No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG9520.1592079737775102No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9470.15837179744464513No Hit
CAGTAATACAGAGCCGTGTCCTCAGGTCTCAGGCTGCTCATTTGTAGATA9430.15770285637835307No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA9390.157033915312061No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9250.1546926215800388No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG9230.15435815104689277No Hit
CTCCTCACTCTTTGCATAGATTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA9210.15402368051374676No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG9030.1510134457154325No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8860.1481704461836912No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8560.14315338818650078No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGGTAACCATCTGGGGGTGGTATTCGGCGGAGGGACCAAGCTGACCGT8430.1409793297210516No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8210.13730015385644526No Hit
GACTATTACTGTAACTCCCGGGACAGCAGTGGTAACCATCTGGGGGTGGT7980.13345374272526592No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7920.13245033112582782No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG7880.13178139005953576No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAC7630.1276005083952104No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7610.12726603786206436No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7540.12609539099605324No Hit
CTCTTTGCATAGATTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT7380.12341962673088501No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC7300.12208174459830089No Hit
GTATTACTGTGCGAAAGAGCATAGCAGCAGCCCCGGGTGGTTCTTTGACTCCTGGGGCCAGGGAACTCTGGTCGC7280.12174727406515486No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG6990.11689745133453744No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGCTCAAGGAAG6880.11505786340223426No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT6850.11455615760251522No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGGTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT6650.11121145227105493No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGCTCAAGGA6490.1085356880058867No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC6420.10736504113987558No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG6330.10585992374071845No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGATTCTGTGGTTTCTTCTGAGCTGACTCAGGA6300.1053582179409994No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6290.10519098267442639No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAACCTATTATGCAAGCTGGTA6150.10284968894240419No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6140.10268245367583116No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC6120.10234798314268513No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTG200.00661518853.16010359
TTATCGA200.00712258552.17599548
GGTATCA6400.047.4525641
GTATCAA15450.037.1048281
TCTGGAG5400.035.1680270
CGAGCGA400.002736676334.12890619
GCTCGGG2105.2023097E-1033.4933570
AATGGTA2156.693881E-1032.71443670
GATAGAG450.00368258932.12211265
GGATGGG4650.031.7646670
GCATATA553.468256E-431.02627419
TATCAAC18650.030.5349062
ATAAACG450.0048919230.3114323
CCGCAGG3101.8189894E-1229.49575670
ATCAACG19200.029.3049963
GTATAAG704.1473213E-529.248451
TCAACGC19450.028.9331674
AAGTACT500.006332549728.75191164
CAACGCA19950.028.2080215
AACGCAG20650.027.912476
Produced by FastQC (version 0.11.5)