FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707065
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27800.39317460205214516No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27130.3836988112832625No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21510.3042153125950231No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18410.2603721015748199No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17940.25372490506530515No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17430.2465119897039169No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16730.2366119097961291No Hit
GACTTACAGTAGCCCTCGCACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCAT16400.23194472926817197No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15830.2238832356289733No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTTTGCAACTTACTACTGTCAACAGA15780.2231760870641313No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15380.21751889854539538No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14390.20351735696152404No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13510.19107154222030506No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12900.1824443297292328No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12750.18032288403470684No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGCGGCTATTTAAATTGGTATCAGCAGCAA12740.18018145432173846No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12370.17494855494190772No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11880.16801849900645627No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11670.1650484750341199No Hit
CTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC11640.16462418589521471No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10870.1537340979966481No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10570.14949120660759618No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10560.14934977689462778No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10340.14623832320932306No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9920.14029827526465036No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGCGG9830.13902540784793477No Hit
GTTCAGTGGCGGTCGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTTTGCAACTTA9820.1388839781349664No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9810.13874254842199799No Hit
ACTTACTACTGTCAACAGACTTACAGTAGCCCTCGCACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAA9700.1371868215793456No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCTGGCCAAAAGTGCGAGGGCTA9610.13591395416263002No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9510.13449965703294606No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCTGGCCAAAAGTG9470.13393393818107246No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGTAGC9200.13011533593092572No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9160.12954961707905213No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8890.1257310148289054No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8740.12360956913437945No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8730.12346813942141104No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8690.12290242056953746No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8680.12276099085656905No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGAGATCCAGATGACCCAGTC8670.12261956114360066No Hit
GGCCAAAAGTGCGAGGGCTACTGTAAGTCTGTTGACAGTAGTAAGTTGCA8600.12162955315282188No Hit
CTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCA8550.1209224045879799No Hit
CTCCTGATCTATGCTGCATCCAGTGTGCAAAGTGGGGTCCCGTCTAGGTT8500.1202152560231379No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8460.11964953717126432No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8420.11908381831939072No Hit
ATGCTGCATCCAGTGTGCAAAGTGGGGTCCCGTCTAGGTTCAGTGGCGGTCGATCTGGGACAGATTTCACTCTCA8330.11781095090267514No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8300.11738666176376994No Hit
GAGTATTAGCGGCTATTTAAATTGGTATCAGCAGCAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATC8250.11667951319892797No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8210.11611379434705436No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8190.11583093492111758No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT7990.11300234066174963No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGAGAT7980.11286091094878123No Hit
GATCTATGCTGCATCCAGTGTGCAAAGTGGGGTCCCGTCTAGGTTCAGTGGCGGTCGATCTGGGACAGATTTCA7950.11243662180987603No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7950.11243662180987603No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7900.11172947324503404No Hit
ACAGTAGCCCTCGCACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGA7820.11059803554128687No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7670.1084765898467609No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7670.1084765898467609No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7590.10734515214301374No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAATTTAAAT7520.10635514415223496No Hit
CCACTGAACCTAGACGGGACCCCACTTTGCACACTGGATGCAGCATAGAT7390.10451655788364578No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7090.10027366649459385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)