FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707065
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27930.3950131883207343No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22110.3127010953731269No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20990.29686096752066643No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20920.2958709595298876No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20880.295305240678014No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16890.23887478520362343No Hit
GACTTACAGTAGCCCTCGCACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCAT16570.23434903438863472No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15040.21271028830446992No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14940.21129599117478592No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTTTGCAACTTACTACTGTCAACAGA14450.20436593523933444No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14200.20083019241512448No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14200.20083019241512448No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13600.19234440963702062No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13120.18555578341453757No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12970.1834343377200116No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12750.18032288403470684No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12640.17876715719205447No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12370.17494855494190772No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12260.17339282809925538No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12100.171129952691761No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGCGGCTATTTAAATTGGTATCAGCAGCAA11850.16759420986755108No Hit
CTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC11020.15585554369117408No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10800.15274409000586933No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10560.14934977689462778No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9990.14128828325542914No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9890.13987398612574517No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGCGG9870.13959112669980836No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCTGGCCAAAAGTG9410.13308535990326206No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCTGGCCAAAAGTGCGAGGGCTA9390.13280250047732528No Hit
GTTCAGTGGCGGTCGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTTTGCAACTTA9340.13209535191248328No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9320.1318124924865465No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9300.13152963306060972No Hit
CTCCTGATCTATGCTGCATCCAGTGTGCAAAGTGGGGTCCCGTCTAGGTT9080.12841817937530495No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGTAGC9030.12771103081046295No Hit
ACTTACTACTGTCAACAGACTTACAGTAGCCCTCGCACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAA8980.12700388224562098No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG8910.1260138742548422No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGAGATCCAGATGACCCAGTC8600.12162955315282188No Hit
CTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCA8570.12120526401391668No Hit
GATCTATGCTGCATCCAGTGTGCAAAGTGGGGTCCCGTCTAGGTTCAGTGGCGGTCGATCTGGGACAGATTTCA8530.1206395451620431No Hit
ATGCTGCATCCAGTGTGCAAAGTGGGGTCCCGTCTAGGTTCAGTGGCGGTCGATCTGGGACAGATTTCACTCTCA8510.12035668573610629No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8320.11766952118970675No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8170.11554807549518079No Hit
GAGTATTAGCGGCTATTTAAATTGGTATCAGCAGCAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATC8030.11356805951362323No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8000.11314377037471803No Hit
GGCCAAAAGTGCGAGGGCTACTGTAAGTCTGTTGACAGTAGTAAGTTGCA7950.11243662180987603No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7910.11187090295800245No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7910.11187090295800245No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT7790.11017374640238167No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7590.10734515214301374No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7480.10578942530036135No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7470.10564799558739296No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7380.10437512817067737No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7360.10409226874474058No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7280.1029608310409934No Hit
GTAGTAAGTTGCAAAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCGACCGCCA7280.1029608310409934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25800.038.4621771
CGAAACC601.1458598E-437.46225769
CCGAAAC701.3902611E-534.32043568
CAACGCA31650.031.3486185
AACGCAG31700.031.3013886
ATCAACG31500.031.2821393
TCAACGC31850.031.1495634
CTCGCTA553.7481703E-430.61909323
GATTCAC1550.030.3828071
TATCAAC32850.030.096822
ACGGGCT902.480392E-729.9111216
CGCAGAG33500.029.619528
ACGCAGA33950.029.2269217
GTAGTTT1703.5543198E-629.21501570
AGAGTAC34150.028.85876511
GCAGAGT35300.028.2044649
GTGGTAT2900.027.8384341
GCCCTAG500.00874869826.9104861
TGTTAGA10450.025.12131970
TGGCGAG700.001480603624.203630
Produced by FastQC (version 0.11.5)