FastQCFastQC Report
Wed 8 May 2024
EGAF00004692427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048781
Total Bases77.4 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT39940.3808230698305938No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39050.37233702746331215No Hit
GTAATATACGGCCGTGTCCGCAGCGGTCACAGAGTTCAGCCTCAGGGAAA37360.35622308184454143No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC31570.301016132061889No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29150.27794172472613443No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTCGAGCCTGAAGATT26540.25305569036815123No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26240.2501952266488428No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25750.24552313590730573No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT25550.24361616009443343No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25180.2400882548406197No Hit
GTATATTACTGTGCGAGAGGGTCGGCTGCCACTTTTCACTACTGGGGCCA23550.22454640196571066No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG22630.2157743132264982No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTACTTAGCCTGGTACCAAC22620.21567896443585458No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT22290.21253245434461532No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21720.20709757327792935No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT21600.20595338779020597No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20970.1999464139796583No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA20610.19651385751648817No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19500.18593014175504707No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC18050.17210456711172303No Hit
GTTTATTACTGTCAGCACCGTAGCAACTGGCAATTCACTTTCGGCCCTGG18030.17191386953043583No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG17790.16962549855498907No Hit
GTAATAAACTGCAAAATCTTCAGGCTCGAGGCTGCTGATGGTGAGAGTGA17720.16895805702048378No Hit
GACTTCACTCTCACCATCAGCAGCCTCGAGCCTGAAGATTTTGCAGTTTA17430.16619294209181898No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17350.16543015176667006No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17250.16447666386023393No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC17020.1622836416754308No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16980.16190224651285637No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA16630.15856503884032988No Hit
GTCTCTGGTGCCTCCATCAGTGATTACTACTGGAGCTGGATCCGTCAGCC16380.15618131907423952No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16330.15570457512102145No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC16210.1545603896332981No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA16200.15446504084265447No Hit
TCTTTGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGC15840.15103248437948436No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCT15690.14960225251983017No Hit
CACCTGCACTGTCTCTGGTGCCTCCATCAGTGATTACTACTGGAGCTGGA15290.14578830089408562No Hit
GTCAGAGTGTTGGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCGG14840.14149760531512298No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA14830.14140225652447938No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT14530.13854179280517095No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG14120.13463249238878278No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13270.12652784518407562No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13230.12614645002150116No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC13120.1250976133244214No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC13120.1250976133244214No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12400.1182325003980812No Hit
ACAGAAACCTGGCCGGGCTCCCAGGCTCCTCATCTTTGATGCATCCAACA12210.11642087337585254No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT12150.11584878063199086No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12120.11556273426006003No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC12010.11451389756298026No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12000.11441854877233665No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCGAGGCTGCTGATGGTGAGAGT11900.1134650608659005No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11640.11098599230916656No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11590.11050924835594848No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11340.10812552858985812No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11340.10812552858985812No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11230.10707669189277838No Hit
CCGTAGCAACTGGCAATTCACTTTCGGCCCTGGGACCAAAGTGGATATCA11100.1058371576144114No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10970.10459762333604442No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10970.10459762333604442No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10620.10126041566351793No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCC10520.1003069277570818No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10490.10002088138515094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)