FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048781
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36460.34764169068661616No Hit
GTAATATACGGCCGTGTCCGCAGCGGTCACAGAGTTCAGCCTCAGGGAAA31700.30225566634025597No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25890.24685801897631632No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24970.23808593023710384No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24940.23779988386517298No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23840.22731151689437548No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTCGAGCCTGAAGATT23060.21987431122417359No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21860.20843245634693994No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG21330.20337897044282838No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20660.19699060146970626No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20310.19365339379717977No Hit
GTATATTACTGTGCGAGAGGGTCGGCTGCCACTTTTCACTACTGGGGCCA17890.17057898646142522No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCGGGCTCCCAGGCT17530.16714642999825513No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT17320.16514410539473923No Hit
GTTTATTACTGTCAGCACCGTAGCAACTGGCAATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA16850.16066271223448939No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16350.15589527270230868No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTACTTAGCCTGGTA16310.15551387753973422No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15560.1483627182414632No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG15550.14826736945081956No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15500.1477906254976015No Hit
GACTTCACTCTCACCATCAGCAGCCTCGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCACCGTAGCAACTGG15500.1477906254976015No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15320.14607434726601645No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14610.13930458313031988No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14540.13863714159581458No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC14500.1382557464332401No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14430.1375883048987348No Hit
GTAATAAACTGCAAAATCTTCAGGCTCGAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14390.13720690973616037No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13970.1332022605291286No Hit
GTCTCTGGTGCCTCCATCAGTGATTACTACTGGAGCTGGATCCGTCAGCC13910.1326301677852669No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13600.12967435527531487No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13590.12957900648467124No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13520.12891156495016595No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC13120.1250976133244214No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA13000.12395342783669804No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12910.12309528872090551No Hit
TCTTTGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT12890.12290459113961827No Hit
CACCTGCACTGTCTCTGGTGCCTCCATCAGTGATTACTACTGGAGCTGGATCCGTCAGCCCCCAGGGAAGGGA12740.12147435927996407No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12640.12052087137352793No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12110.11546738546941639No Hit
GTCAGAGTGTTGGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCGGGCTCCCAGGCTCCTCATCTTTGATG11860.11308366570332605No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11730.11184413142495907No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11650.11108134109981016No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11140.10621855277698586No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT11050.10536041366119332No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10630.10135576445416154No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10600.10106971808223071No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCCG200.00422027359.55678668
GGTATCA10300.044.7273251
TACTGAT11850.042.678270
GTATCAA24750.036.269531
AATGGTA4700.030.7438770
ATCAACG29800.029.7594453
TATCAAC29950.029.7262522
TCAACGC30350.029.335874
CAACGCA30950.029.098085
AACGCAG31650.028.776816
GACGAGT10300.028.32606162
GTCTACT10100.028.26710367
TCTACTG10900.028.04815168
TGTTAGA11400.027.88523170
ATACGTA1104.397043E-827.70085711
CTACTGA12950.027.67130769
AGAGTAC32800.027.56009911
CGCAGAG33100.027.5161958
ACGAGTC10400.027.4571563
ACGCAGA33200.027.4333137
Produced by FastQC (version 0.11.5)