FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465463
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11560.24835486386673056No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8840.18991842530985278No Hit
GTATATTACTGTCAGAGGTTTAGTGACCCAATGGCCATTTTTGGCCCGGGGACCAGGCTGGAGATCAAGCGAA7360.1581221278597869No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT7310.15704792862160902No Hit
GTAATATACTGCAGAATCTTCGGGCTCAACTCTGGTGATGGTGAGAGTGAAGTCTGTTCCGGACACACTGCCA6720.1443723776111098No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT6580.1413646197442117No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG6250.13427490477223752No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT6190.13298586568642404No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC5980.12847422888607687No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT5800.12460711162863643No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5010.10763476366542561No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAA4970.10677540427488329No Hit
CTCTTGCAGGGCCAACTGGCATATCGCCGACATCACCTTAGCCTGGTACC4840.10398248625562075No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC4790.10290828701744285No Hit
ATATTACTGTCAGAGGTTTAGTGACCCAATGGCCATTTTTGGCCCGGGGA4760.1022637674745361No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)