FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357069
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT41260.30403759867773855No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC38400.28296276755271843No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT30590.22541226717285565No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT29090.21435903406532755No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC23510.17324100690532315No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG23160.1706619191802333No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23130.17044085451808272No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22180.16344047354998162No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC21110.15555583393327826No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG21000.15474526350539286No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20840.1535662519739232No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19300.14221826598352771No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18920.13941811359628728No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18690.13772328451979965No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG18560.13676533765048055No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18510.1363968965468963No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA18410.13566001433972774No Hit
ATGCTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCGGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG17570.12947020379951202No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT16670.1228382639349952No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16580.12217506994854352No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACGCCACTCAGA16170.11915385289915251No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15400.11347985990395477No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC15030.11075339573743119No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15020.11067970751671433No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14810.1091322548816604No Hit
CCTTCGCTGTGACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAG14600.10758480224660648No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACGCCACTCAGACTGCTGTCATAGGA14030.10338457366574581No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGGTGTACACTGGTACCAGCACCTTCCAGGAACAGC13740.10124761526495706No Hit
GACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGC13600.10021598017492109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT408.490406E-751.54989239
GTCGTAC200.0080766350.5466731
TCGTATG4450.045.68146540
CGTATGC4650.045.2941841
GGTATCA20250.043.7655141
TGTCGTA551.5438854E-743.63596338
ATCTCGT3900.042.10898637
GCGTATG350.001356633739.37676240
GCCGTCT4650.039.08525546
GTATCAA56150.038.768951
ATGCCGT5150.038.5072444
ATCTCCG5600.038.49756210
ATGTCGT557.830622E-637.3238737
CACGAGA5900.037.12467621
TAGGCAT5900.036.6901129
CCCACGA5900.036.54544419
CGTCTTC4950.036.1707748
CCGAAAC1102.419256E-1035.98166768
GTATGCC6100.035.7459842
CCGTCTT5100.035.70890847
Produced by FastQC (version 0.11.5)