FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078977
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36040.33402009496031887No Hit
GTGTAGCAACTGGCCTATTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT33540.3108499995829383No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27100.2511638338908058No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT25540.23670569437532035No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23790.22048662761115387No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23170.21474044395756348No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGACCCCAGCTTCAGGGAGA22850.21177467174925876No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAG22630.20973570335604932No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC22330.20695529191076362No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22120.20500900389906362No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21190.19638972841867805No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19680.18239499081074018No Hit
GTTGCTACACTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT19270.17859509516884975No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTGTAGCAAC18070.16747344938770706No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGTCAGTGGCCCTGTT17910.1659905632835547No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17550.16265406954921188No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCTACTTAGCCTGGTACCAACAGAAACGTGGCCAGGCTCCCAGGCT17140.15885417390732148No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16350.1515324237680692No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16150.14967881613787876No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16000.14828861041523592No Hit
GTTTATTACTGTCAGCAGTGTAGCAACTGGCCTATTTTTGGCCAGGGGAC15720.14569355973296927No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15430.14300582866919315No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15260.14143026218353125No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15180.1406888191314551No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14800.13716696463409322No Hit
GTGTATTACTGTGCGAGGTCATATTACTATGACAGTAGTGGTTGGGCCCCTTTTGGCTACTGGGGCCAGGGAACC14610.1354060373854123No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAGCTGGATCCG14380.13327438861069327No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14080.13049397716540762No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTGTAGCAACTGG14010.12984521449484093No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCTACTTAGCCTGGTA13800.12789892648314097No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13630.1263233599974791No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13620.12623067961596957No Hit
TCTATGATGCATCCAACAGGGCCACTGACATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT13610.12613799923446006No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13110.12150398015898392No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12540.11622119841294115No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG12310.11408954963822214No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAATA12120.1123286223895412No Hit
TTGCTACACTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC12080.11195790086350312No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11820.10954821094425553No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT11730.10871408751066984No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11550.10704584064349842No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGTCA11450.10611903682840321No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11410.10574831530236511No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC10870.1007435747008509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2100.049.2301841
TCGTATG2100.049.14685440
ATCGTAT306.450167E-445.7971939
GGTATCA10900.044.1888351
ATGCCGT2300.040.6438644
ACGAGAC2950.040.24545722
CTTATAC3050.040.0362471
CACGAGA3000.038.4404321
GAGACCG3050.037.81902724
ACCGAGG3150.037.75144227
ATCTCGT1850.037.03376837
CCCACGA3150.036.60314619
GTATGCC2900.035.7066142
GACCGAG3250.035.5246426
AGACCGA3250.035.50486825
ATCTCCG3450.035.38451810
TCTCCGA3450.035.38451811
TTATACA3750.035.2714772
TATACAC3750.035.2714773
CCGTCTT2800.034.81527747
Produced by FastQC (version 0.11.5)