FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078977
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37160.3444002976893854No Hit
GTGTAGCAACTGGCCTATTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT33140.30714278432255737No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26370.2443981660406107No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24430.22641817202776332No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT23540.21816961807341584No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22890.21214539327529688No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22470.20825281725189693No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21780.2018578709277399No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21780.2018578709277399No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGACCCCAGCTTCAGGGAGA21710.20120910825717322No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20360.18869725675338772No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20020.18554612378206395No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAG19970.18508272187451633No Hit
GTTGCTACACTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT19230.17822437364281166No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17560.1627467499307214No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCTACTTAGCCTGGTACCAACAGAAACGTGGCCAGGCTCCCAGGCT17100.1584834523812834No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTGTAGCAAC16590.15375675292429775No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGTCAGTGGCCCTGTT16140.14958613575636923No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15430.14300582866919315No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15270.14152294256504078No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAGCTGGATCCG15220.14105954065749315No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15190.1407814995129646No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15170.14059613874994556No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTGTAGCAACTGG14900.13809376844918844No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14880.13790840768616938No Hit
GTTTATTACTGTCAGCAGTGTAGCAACTGGCCTATTTTTGGCCAGGGGAC14590.13522067662239326No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14480.1342011924257885No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14280.13234758479559805No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14170.1313281005989933No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCTACTTAGCCTGGTA14140.13105005945446474No Hit
GTGTATTACTGTGCGAGGTCATATTACTATGACAGTAGTGGTTGGGCCCCTTTTGGCTACTGGGGCCAGGGAACC13910.12891841067974572No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13800.12789892648314097No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13060.12104057825143631No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12810.11872356871369825No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12180.11288470467859833No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG12040.11158717933746504No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11890.1101969736148222No Hit
TTGCTACACTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC11870.11001161285180315No Hit
TCTATGATGCATCCAACAGGGCCACTGACATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT11810.10945553056274601No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGTCA11640.10787996407708413No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11430.10593367606538415No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC11420.10584099568387464No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAATA11350.10519223301330798No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT10800.10009481203028424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8550.040.4466361
GACGGTA350.001365869139.3217340
TCGCTTA350.001469352738.74180616
TACGTAT750.001540058438.36913370
GTATCAA24650.037.273551
TATCAAC26650.033.951662
GCGTGAA850.002846496233.85511870
ATCAACG27700.032.780943
TCAACGC28200.032.1982274
TACTGAT11250.031.9742870
CAACGCA28600.031.7479065
AACGCAG28900.031.418346
AATGGTA6700.031.1391370
GAATCCG450.00493988530.25318130
TATACCC450.00504996130.1171533
CGATTAA703.43527E-530.07022551
CGCAGAG30400.029.9795448
ACGCAGA30650.029.7350127
GCAGAGT31350.029.071079
AGAGTAC31550.028.88678611
Produced by FastQC (version 0.11.5)