FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373093
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42980.31301594283854045No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG41870.30493200387737757No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGGCTGTCATCCCATG35560.25897735987292925No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33460.24368342129775625No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC32150.23414291675800547No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29120.21207594824239873No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28150.2050116051862474No Hit
GATTATTCCTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGG27620.20115170640298946No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27610.20107887812406008No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26730.19466998957827328No Hit
GGAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG26570.19350473711540297No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGG26440.19255796948932083No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC25510.18578493954888708No Hit
GTAATACACGGCCATGTCCGCAGCGGTCACAGAGCTCAACTTCAGGGAGAACTGGTTCTTGGACGTGACTACTGA24220.1763900915669951No Hit
GATTATTACTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGG23960.17449655631483083No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23560.171583425157655No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC23210.1690344353951262No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT23180.16881595055833798No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG22670.16510170833293883No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21390.15577968862997627No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG21330.1553427189563999No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA20840.15177413328885953No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT20400.14856968901596615No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20270.147622921389884No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC20230.14733160827416644No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19990.14558372957986093No Hit
ATACCACACCATTCAGGCTGTCATCCCATGCTGCACAGGAATAATCAGCC19720.1436173660487673No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19440.14157817423874422No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19390.14121403284409723No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18830.13713564922405108No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18710.13626170987689837No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18320.13342140699865193No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17930.13058110412040555No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17730.12912453854181766No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17730.12912453854181766No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC17680.12876039714717064No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC17180.12511898320070092No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG17040.12409938729568938No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16410.11951120572313748No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16360.1191470643284905No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT16240.11827312498133774No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC16080.11710787251846744No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGGC15890.11572413521880892No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC15870.11557847866095013No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15710.11441322619807981No Hit
GTCTCTGGTGGCTCCATCAGTGGTCACTACTGGAGCTGGATCCGGCAGCC15540.11317514545628009No Hit
CCCCTGAGGGCCGCTGATTATTACTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGCTTA15210.11077181225161005No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT15090.1098978729044573No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14820.10793150937336365No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14590.10625645895798756No Hit
GTCTGAGGATGAGGCTGATTATTCCTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGAC14410.10494554993725844No Hit
GGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTCGGACATGACCAGGGA14130.10290635812723538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT253.156856E-668.7693439
CGTATGC3600.050.81237441
TCGTATG3500.049.20680640
TAACGCC1250.048.5833744
TCTCGTA3150.047.93507438
GGTATCA19050.046.9464341
CTAACGC1150.046.942173
ATGCCGT3850.046.91003844
ACATGCG4300.046.3869127
GATCTCG2900.045.9514836
ATGCGCA4350.045.9241229
GGATGGG6150.045.83165770
TGCCGTC3800.045.79717645
GTATGCC4100.045.5679142
CTTATAC4550.045.2449571
GCCGTCT3550.045.18713846
CCTAACG1200.044.9862482
ATCTCGT3000.044.4969137
CGAGACA4650.044.27194623
CATGCGC4550.043.868628
Produced by FastQC (version 0.11.5)