FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373093
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41230.3002709940258963No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG38430.27987907592566563No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37530.2733245308220201No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC34560.2516945319799897No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33090.24098877497736862No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGGCTGTCATCCCATG31890.2322493815058412No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30840.22460241221825472No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29390.2140423117734924No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27480.2001321104979779No Hit
GATTATTCCTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGG26440.19255796948932083No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT26070.1898633231689332No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25160.18323594978635824No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24180.17609877845127753No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24140.17580746533555994No Hit
GTAATACACGGCCATGTCCGCAGCGGTCACAGAGCTCAACTTCAGGGAGAACTGGTTCTTGGACGTGACTACTGA23800.1733313038519605No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22630.16481039521722127No Hit
GATTATTACTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGG22380.16298968824398638No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG21940.159785243971093No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC21740.15832867839250508No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA21540.1568721128139172No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGG21530.15679928453498782No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC21460.15628948658248204No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21130.153886153377812No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21100.1536676685410238No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20950.15257524435708286No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20600.15002625459455404No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC20220.147258779995237No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT19780.14405433572234363No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC19700.14347170949090848No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19480.14186948735446178No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18140.13211049797792282No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18070.13160070002541707No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17920.13050827584147615No Hit
GGAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG17390.1266483770582182No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17250.12562878115320666No Hit
ATACCACACCATTCAGGCTGTCATCCCATGCTGCACAGGAATAATCAGCC17230.12548312459534788No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16930.12329827622746602No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16850.12271564999603086No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16320.11885575121277292No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC16090.11718070079739683No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT16080.11710787251846744No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC15830.11528716554523254No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15800.11506868070844437No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG15790.11499585242951497No Hit
CCCCTGAGGGCCGCTGATTATTACTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGCTTA15680.11419474136129162No Hit
GTCTGAGGATGAGGCTGATTATTCCTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGAC14880.10836847904694001No Hit
GTCTCTGGTGGCTCCATCAGTGGTCACTACTGGAGCTGGATCCGGCAGCC14270.10392595403224691No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA14140.10297918640616477No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14130.10290635812723538No Hit
GCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG13970.10174110566436505No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT13970.10174110566436505No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13910.10130413599078868No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13880.10108565115400049No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13780.10035736836470655No Hit
CCCAGGAACGGCCCCCAAACTCCTCATGTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG13750.10013888352791835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGA200.00544414755.8475166
CTAACGC850.055.5197263
CCTAACG1000.047.19522
GGATGGG8850.045.89994470
GGTATCA20150.042.8645971
TAACGCC1150.041.0393074
GTATCAA49850.037.9015241
CGACTAG400.001988674336.427162
CCGAGTT751.1844368E-633.64633659
TATCAAC58850.031.849172
ATCAACG60250.031.0508963
TCAGGTC6700.030.68862212
TCAACGC61100.030.6211574
AACGCAG61850.030.4111376
AGGATGG8800.030.22795969
CAACGCA62200.030.076345
GTCATAG7400.029.6357211
CGCAGAG64250.029.1702298
ACGCAGA65100.028.7893567
TAGGTGG7400.028.6966515
Produced by FastQC (version 0.11.5)