FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467871
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTATAGTGCCCCTCTCAGTTTCGGCGGCGGGACCAAGGTGCAGATCAAACGAACTGTGGCTGCACCAT51770.3526876680580242No Hit
CTATAACTCTGCTGACAATAGTAAGTCGCAAAATCCTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTG49170.3349749398959445No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39120.2665084329617521No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37980.2587420829214556No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29010.19763317076228087No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28610.19490813566042248No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28520.19429500276250433No Hit
GTAATAGACAGCCGTGTCCTCGGTTCTCAGACCATTCATCTGCAGAAACA26850.1829179812122455No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24140.1644558683971548No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGAATATTGGAAC23170.15784765827514816No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC22950.15634888896912605No Hit
GTCTATTACTGTACGACCGGACGCAGAACCTATTATTATGATGATACTGGTCACGGGTATTTTGACTACTGGGGC22750.15498637141819685No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22440.15287446921425657No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21780.1483781612961902No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21270.14490374154132074No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20090.13686488799083843No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19490.13277733533805083No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGAATATTGGAAC19150.1304610555014712No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19040.1297116708484601No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19040.1297116708484601No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18940.12903041207299554No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGGCGTCCAGATGACCCAGTC17970.12242220195098888No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17830.12146843966533843No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17810.12133218791024551No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17680.12044655150214154No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17600.11990154448176986No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17350.11819839754310835No Hit
GATTTCACTCTCACCATCAGCGGTCTGCAACCTGAGGATTTTGCGACTTACTATTGTCAGCAGAGTTATAGTGCC16820.11458772603314597No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16710.1138383413801349No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16700.11377021550258845No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16290.11097705452318357No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15760.1073663830132212No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15620.10641262072757075No Hit
GAGTGAAATCTGTGCCAGATCCACTGCCACTGAACCTGGATGGGACCCCA15460.10532260668682739No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15400.10491385142154862No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15190.10348320799307296No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15100.10287007509515482No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)