FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467871
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTATAGTGCCCCTCTCAGTTTCGGCGGCGGGACCAAGGTGCAGATCAAACGAACTGTGGCTGCACCAT52270.35609396193534715No Hit
CTATAACTCTGCTGACAATAGTAAGTCGCAAAATCCTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTG46230.31494593189728526No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39790.2710728667573649No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38550.26262525794160385No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31670.2157546541896393No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30000.20437763263938044No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27810.18945806545670568No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27150.18496175753863928No Hit
GTAATAGACAGCCGTGTCCTCGGTTCTCAGACCATTCATCTGCAGAAACA23220.15818828766288046No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23170.15784765827514816No Hit
GTCTATTACTGTACGACCGGACGCAGAACCTATTATTATGATGATACTGGTCACGGGTATTTTGACTACTGGGGC22790.15525887492838267No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22120.15069444113276984No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20960.14279183933738046No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20640.14061181125589375No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGAATATTGGAAC20270.13809115378667472No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20260.13802302790912824No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19500.1328454612155973No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGAATATTGGAAC18940.12903041207299554No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18810.12814477566489155No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18570.12650975460377648No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17690.12051467737968799No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17260.1175852646451902No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16980.11567774007388934No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16690.11370208962504198No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16670.11356583786994907No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16590.11302083084957738No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16480.11227144619656632No Hit
GTGCAGCCACAGTTCGTTTGATCTGCACCTTGGTCCCGCCGCCGAAACTG16400.11172643917619464No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16180.1102276698701725No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15880.1081838935437787No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGGCGTCCAGATGACCCAGTC15750.10729825713567474No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15620.10641262072757075No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15610.1063444948500243No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15010.10225694219723669No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15000.10218881631969022No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14970.10198443868705083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG12600.034.29263770
GTATCAA34100.033.1844371
AATGGTA10700.031.11398370
GTGGTAT4550.030.3474371
TACGTAT950.005295345629.8248370
GAATACG2750.029.3789089
ATACGTA2750.029.37690511
ATCAACG38900.029.1613643
TCAACGC39100.029.0963064
GTGCTAG2900.029.0329781
TATCAAC38900.028.7326162
CAACGCA39950.028.4801485
AACGCAG39900.028.432446
CGTACCA2900.027.84982114
ACGTACC2900.027.84982113
CCGGACC500.007584646327.71163643
CGCAGAG41900.027.2359668
CTAGAGA3100.027.1441484
AGAGTAC42650.026.59735111
TACGTAC3050.026.48647112
Produced by FastQC (version 0.11.5)