FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682992
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC67550.40136851512068983No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63780.3789679332997424No Hit
GTAATACACAGCCGTGTCGTCAGGTCCCAGGGCGTTCATTTGCAGATGCA49530.29429729909589586No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48820.2900786218829323No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC43940.26108264329242203No Hit
GAGTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGACAGCCCCTAAACTCCTGATCTATAAGGCAT41490.24652523600825196No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA39790.2364241778927054No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38030.2259666118436689No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36170.21491486590548264No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36080.21438010400524782No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35210.20921073896964454No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG33790.20077338454371738No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32840.19512867559679428No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG31900.18954338463878617No Hit
CAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTCTGCAACTTA31010.18425518362535295No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30480.181106030212859No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30290.17997708842347437No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29420.17480772338787112No Hit
GTTTAGGGGCTGTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACCA28400.16874708851854317No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28350.16844999857396828No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28320.16827174460722333No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28310.16821232661830834No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAATTG28100.16696454885109377No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28050.16666745890651888No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26980.160309734092616No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCC26730.1588242843697415No Hit
GTGTATTACTGTGCGAAACGGTCACAGGCCTGGAACGAGAAATTGGACTG26570.1578735965471018No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT26300.15626931084639736No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24400.14497989295255118No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23800.14141481361765237No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23710.14088005171741755No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23230.13802798824949852No Hit
GCCCCTAAACTCCTGATCTATAAGGCATCTATTTTACAAGGTGGGGTCCCATCGAGGTTCAGCGGCAGTGGAT21930.1303036496905511No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21660.12869936398984666No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21590.1282834380674418No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20940.1244212687879681No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20940.1244212687879681No Hit
CCAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGA20720.12311407303183854No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20370.12103444341981424No Hit
GTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGACAGCCCCT20110.11948957570802475No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19840.1178852900073203No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTCTGCAACTTATTTCTGCCAACAGTATTCTATTTTTACCAATTTCGG19750.11735052810708547No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAA19350.11497380855048628No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19120.11360719480544174No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC19070.11331010486086684No Hit
CAGTAATACACAGCCGTGTCGTCAGGTCCCAGGGCGTTCATTTGCAGATG18610.11057687737077776No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGGTAG18580.11039862340403281No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18470.10974502552596804No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC18090.1074871419471988No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17850.10606111021323927No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17820.10588285624649435No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17690.10511042239059959No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17530.1041597345679599No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17490.1039220626123No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17450.10368439065664006No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCAAGCCATAATTAT17390.10332788272315019No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17350.10309021076749028No Hit
GTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACCAATACTCTGACTGGCCCGGCAAGTGATGGTGACT17350.10309021076749028No Hit
GACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCT17330.10297137478966031No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17290.1027337028340004No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16910.10047581925523116No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC16880.10029756528848621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT907.2759576E-1267.7873770
TGGTACG659.458745E-1151.88726867
GTCGTAT352.3156417E-549.1322339
ACATGCG2700.042.8385127
CGTATGC2350.039.6317641
GTACGTA1001.3442332E-939.61178669
GGTACGT901.4624675E-939.27487668
TCTGGAG16500.039.02909570
ATCTCCG3250.037.60289410
ATGCGCA3100.037.34765229
TCGTATG2500.037.21004540
GTATCAA39050.037.046361
ATCTCGT2050.036.791737
CGAGACA3500.035.9199723
TATACAC3800.035.737993
GCGCAGA3350.035.63009631
CATGCGC3250.035.60163528
TCTCCGA3550.035.38144311
ACGAGAC3500.034.94188322
CACGAGA3650.034.4325521
Produced by FastQC (version 0.11.5)