FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993081
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGGAGATACA37470.37731061212529493No Hit
GTATATTACTGTGCGAGGGAAAATGGATATAGTTATGGCTCTCCCTACCT31900.32122253874558065No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24620.24791532614157355No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22920.23079688363789055No Hit
CCTGATATATCGTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTT19070.1920286462030791No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19000.1913237691588098No Hit
ATATATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTACACAACTGGTAACGCTCTGACTAGC18090.18216036758330892No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16790.1690697939040219No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16270.16383356443230712No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15900.16010778576974083No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15550.15658340054839434No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14700.14802417929655284No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14070.14168028589812914No Hit
GTCTACAGCCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTGCATT13830.13926356460349157No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13730.13825659739739257No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13700.13795450723556288No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13420.13513499905848567No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13290.13382594169055695No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13080.13171131055774907No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13050.13140922039591937No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12520.12607229420359467No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT12210.12295069586468778No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12160.12244721226163827No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12040.12123885161431948No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11900.11982909752578089No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC11730.11811725327541259No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11460.11539844181894528No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11450.11529774509833539No Hit
TTCTTGGAGGGGTAATGCAACTCTGTTGACAGTAGTAAGTTGCAAAATCT11140.11217614675942848No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10820.10895385169991169No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTCCCTATGCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG10820.10895385169991169No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10750.1082489746556424No Hit
GAGTTGCATTACCCCTCCAAGAACGTTCGGCCAAGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT10690.107644794331983No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10580.1065371304052741No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10520.1059329500816147No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG10430.10502667959612559No Hit
CCTTGGTCCCTTGGCCGAACGTTCTTGGAGGGGTAATGCAACTCTGTTGA10300.1037176222281969No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10050.1012002042129494No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCTAGTCAGA9960.1002939337274603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)